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Journal of Bacteriology, October 2000, p. 5611-5614, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bacillus subtilis ccpA Gene Mutants Specifically Defective in Activation of Acetoin Biosynthesis

Andrew J. Turinsky,1 Tessa R. Moir-Blais,2 Frank J. Grundy,2 and Tina M. Henkin2,*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210,2 and Department of Biochemistry and Molecular Biology, Albany Medical College, Albany, New York 122081

Received 16 September 1999/Accepted 6 July 2000

A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is involved in acetoin biosynthesis, were identified. These mutants retained normal glucose repression of amyE, encoding alpha -amylase, and acsA, encoding acetyl-coenzyme A synthetase, and normal activation of ackA, which is involved in acetate excretion; in these ccpA mutants the CcpA functions of activation of the acetate and acetoin excretion pathways appear to be separated.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210. Phone: (614) 688-3831. Fax: (614) 292-8120. E-mail: henkin.3{at}osu.edu.

Journal of Bacteriology, October 2000, p. 5611-5614, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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