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Journal of Bacteriology, January 2000, p. 320-326, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation of the MIG1 Gene from Candida albicans and Effects of Its Disruption on Catabolite Repression

Oscar Zaragoza, Cristina Rodríguez, and Carlos Gancedo*

Instituto de Investigaciones Biomédicas "Alberto Sols," Consejo Superior de Investigaciones Científicas-UAM, Unidad de Bioquímica y Genética de Levaduras, 28029 Madrid, Spain

Received 7 July 1999/Accepted 25 October 1999

We have cloned a Candida albicans gene (CaMIG1) that encodes a protein homologous to the DNA-binding protein Mig1 from Saccharomyces cerevisiae (ScMig1). The C. albicans Mig1 protein (CaMig1) differs from ScMig1, in that, among other things, it lacks a putative phosphorylation site for Snf1 and presents several long stretches rich in glutamine or in asparagine, serine, and threonine and has the effector domain located at some distance (50 amino acids) from the carboxy terminus. Expression of CaMIG1 was low and was similar in glucose-, sucrose-, or ethanol-containing media. Disruption of the two CaMIG1 genomic copies had no effect in filamentation or infectivity. Levels of a glucose-repressible alpha -glucosidase, implicated in both sucrose and maltose utilization, were similar in wild-type or mig1/mig1 cells. Disruption of CaMIG1 had also no effect on the expression of the glucose-repressed gene CaGAL1. CaMIG1 was functional in S. cerevisiae, as judged by its ability to suppress the phenotypes produced by mig1 or tps1 mutations. In addition, CaMig1 formed specific complexes with the URS1 region of the S. cerevisiae FBP1 gene. The existence of a possible functional analogue of CaMIG1 in C. albicans was suggested by the results of band shift experiments.

* Corresponding author. Mailing address: Instituto de Investigaciones Biomédicas, C/Arturo Duperier no. 4, E-28029 Madrid, Spain. Phone: 34-91-5854620. Fax: 34-91-5854587. E-mail: cgancedo{at}iib.uam.es.

Journal of Bacteriology, January 2000, p. 320-326, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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