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Journal of Bacteriology, January 2000, p. 508-512, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The C-Terminal Portion of the Tail Fiber Protein of Bacteriophage Lambda Is Responsible for Binding to LamB, Its Receptor at the Surface of Escherichia coli K-12

Jiang Wang,1 Maurice Hofnung,1,* and Alain Charbit2,*

Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA1444, Institut Pasteur, 75724 Paris Cedex 15,1 and Unité INSERM U411, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15,2 France

Received 6 August 1999/Accepted 12 October 1999

Bacteriophage lambda  adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda , to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residues of J could bind to LamB trimers and inhibited recognition by anti-LamB antibodies. Electron microscopy further demonstrated that the fusion protein could also bind to LamB at the surface of intact cells. This interaction prevented lambda  adsorption but affected only partially maltose uptake.

* Corresponding author. Mailing address for Alain Charbit: Unité INSERM U411, Faculté de Médecine Necker-Enfants Malades, 156 rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: 33 1 40 61 53 76. Fax: 33 1 40 61 55 92. E-mail: charbit{at}necker.fr. Mailing address for Maurice Hofnung: Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA1444, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 88 30. Fax: 33 1 45 68 88 34. E-mail: mhofnung{at}pasteur.fr.

Journal of Bacteriology, January 2000, p. 508-512, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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