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Journal of Bacteriology, January 2000, p. 540-542, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The HTR1 Gene Is a Dominant Negative Mutant Allele of MTH1 and Blocks Snf3- and Rgt2-Dependent Glucose Signaling in Yeast

Frank Schulte, Roman Wieczorke, Cornelis P. Hollenberg, and Eckhard Boles*

Institut für Mikrobiologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany

Received 9 August 1999/Accepted 25 October 1999

Saccharomyces cerevisiae HTR1 mutants are severely impaired in the uptake of glucose. We have cloned dominant HTR1 mutant alleles and show that they encode mutant forms of the Mth1 protein. Mth1 is shown to be involved in carbon source-dependent regulation of its own, invertase and hexose transporter gene expression. The mutant forms block the transduction of the Snf3- and Rgt2-mediated glucose signals upstream of the Rgt1 transcriptional regulator.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstr. 1, Geb. 26.12.01, D-40225 Düsseldorf, Germany. Phone: 49 211 81 12778. Fax: 49 211 81 15370. E-mail: boles{at}uni-duesseldorf.de.

Journal of Bacteriology, January 2000, p. 540-542, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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