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Journal of Bacteriology, October 2000, p. 5653-5662, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

Gilles P. van Wezel,1,* Jannes van der Meulen,2 Shinichi Kawamoto,3 Ruud G. M. Luiten,4 Henk K. Koerten,2 and Barend Kraal1

Department of Biochemistry, Leiden Institute of Chemistry, Leiden University,1 and Center for Electron Microscopy, Leiden University Medical Center,2 2300 RA Leiden, and DSM Anti-Infectives, 2600 MA Delft,4 The Netherlands, and National Food Research Institute, Tsukuba City, Japan3

Received 31 March 2000/Accepted 26 June 2000

The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.

* Corresponding author. Mailing address: Department of Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. Phone: (31) 71 5274310. Fax: (31) 71 5274340. E-mail: g.wezel{at}chem.leidenuniv.nl.

Journal of Bacteriology, October 2000, p. 5653-5662, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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