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Journal of Bacteriology, October 2000, p. 5799-5806, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of the ccpA Gene of Enterococcus faecalis: Identification of Starvation-Inducible Proteins Regulated by CcpA

Céline Leboeuf,* Laurence Leblanc, Yanick Auffray, and Axel Hartke

Unité de Microbiologie de l'Environnement, Unité soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France

Received 30 May 2000/Accepted 26 July 2000

Inactivation of ccpA in Enterococcus faecalis leads to reduction of the growth rate, derepression of the galKETR operon in the presence of a mixture of glucose and galactose, and reduction of transcription of ldh in the presence of glucose. Moreover, the E. faecalis ccpA gene fully complements a Bacillus subtilis ccpA mutant, arguing for similar functions of these two homologous proteins. Protein comparison on two-dimensional gels from the wild-type cells and the ccpA mutant cells revealed a pleiotropic effect of the mutation on gene expression. The HPr protein of the carbohydrate-phosphotransferase system was identified by microsequencing, and a modification of its phosphorylation state was observed between the wild-type and the mutant strains. Moreover, at least 16 polypeptides are overexpressed in the mutant, and 6 are repressed. Interestingly, 13 of the 16 polypeptides whose synthesis is enhanced in the mutant were also identified as glucose starvation proteins. The N-terminal amino acid sequences of four of them match sequences deduced from genes coding for L-serine dehydratase, dihydroxyacetone kinase (two genes), and a protein of unknown function from Deinococcus radiodurans.

* Corresponding author. Mailing address: Unité de Microbiologie de l'Environnement, Unité soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France. Phone: (33)-2-31-56-59-30. Fax: (33)-2-31-56-53-11. E-mail: phdlme{at}ibba.unicaen.fr.

Journal of Bacteriology, October 2000, p. 5799-5806, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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