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Journal of Bacteriology, November 2000, p. 5939-5947, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of TnrA in Nitrogen Source-Dependent Repression of Bacillus subtilis Glutamate Synthase Gene Expression

Boris R. Belitsky,1 Lewis V. Wray Jr.,2 Susan H. Fisher,2 Dian E. Bohannon,1,dagger and Abraham L. Sonenshein1,*

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 021182

Received 19 May 2000/Accepted 22 July 2000

Synthesis of glutamate, the cell's major donor of nitrogen groups and principal anion, occupies a significant fraction of bacterial metabolism. In Bacillus subtilis, the gltAB operon, encoding glutamate synthase, requires a specific positive regulator, GltC, for its expression. In addition, the gltAB operon was shown to be repressed by TnrA, a regulator of several other genes of nitrogen metabolism and active under conditions of ammonium (nitrogen) limitation. TnrA was found to bind directly to a site immediately downstream of the gltAB promoter. As is true for other genes, the activity of TnrA at the gltAB promoter was antagonized by glutamine synthetase under certain growth conditions.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6761. Fax: (617) 636-0337. E-mail address: linc.sonenshein{at}tufts.edu.

dagger Present address: 245 Waverley Ave., Newton, MA 02458.

Journal of Bacteriology, November 2000, p. 5939-5947, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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