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Journal of Bacteriology, November 2000, p. 6264-6267, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of the DNA- and Metal-Binding Properties of Vibrio anguillarum Fur Reveals Conservation of a Structural Zn2+ Ion

Ekaterina E. Zheleznova,1 Jorge H. Crosa,2 and Richard G. Brennan1,*

Department of Biochemistry and Molecular Biology1 and Department of Microbiology and Immunology,2 Oregon Health Sciences University, Portland, Oregon 97201-3098

Received 4 February 2000/Accepted 4 August 2000

The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn2+, Co2+, Cd2+, and to a lesser extent Ni2+ but, unlike E. coli Fur, not in the presence of Zn2+. We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, L224, 3181 SW Sam Jackson Park Rd., Portland, OR 97201. Phone: (503) 494-4427. Fax: (503) 494-8393. E-mail: brennanr{at}ohsu.edu.

Journal of Bacteriology, November 2000, p. 6264-6267, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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