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Journal of Bacteriology, December 2000, p. 6550-6556, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Azotobacter vinelandii mucA and mucC Gene Products in Alginate Production

Cinthia Núñez, Renato León, Josefina Guzmán, Guadalupe Espín, and Gloria Soberón-Chávez*

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62251, Mexico

Received 9 May 2000/Accepted 12 September 2000

Azotobacter vinelandii produces the exopolysaccharide alginate, which is essential for its differentiation to desiccation-resistant cysts. In different bacterial species, the alternative sigma factor sigma E regulates the expression of functions related to the extracytoplasmic compartments. In A. vinelandii and Pseudomonas aeruginosa, the sigma E factor (AlgU) is essential for alginate production. In both bacteria, the activity of this sigma factor is regulated by the product of the mucA, mucB, mucC, and mucD genes. In this work, we studied the transcriptional regulation of the A. vinelandii algU-mucABCD gene cluster, as well as the role of the mucA and mucC gene products in alginate production. Our results show the existence of AlgU autoregulation and show that both MucA and MucC play a negative role in alginate production.

* Corresponding author. Mailing address: Departamento de Microbiología Molecular, Instituto de Biotecnología, UNAM, Apdo Postal 510-3, Cuernavaca, Morelos 62251, Mexico. Phone: (52) (73) 291634. Fax: (52) (73) 172388. E-mail: gloria{at}ibt.unam.mx.

Journal of Bacteriology, December 2000, p. 6550-6556, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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