This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Winstedt, L.
Right arrow Articles by von Wachenfeldt, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Winstedt, L.
Right arrow Articles by von Wachenfeldt, C.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2000, p. 6557-6564, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Terminal Oxidases of Bacillus subtilis Strain 168: One Quinol Oxidase, Cytochrome aa3 or Cytochrome bd, Is Required for Aerobic Growth

Lena Winstedt and Claes von Wachenfeldt*

Department of Microbiology, Lund University, Lund, Sweden

Received 26 June 2000/Accepted 8 September 2000

The gram-positive endospore-forming bacterium Bacillus subtilis has, under aerobic conditions, a branched respiratory system comprising one quinol oxidase branch and one cytochrome oxidase branch. The system terminates in one of four alternative terminal oxidases. Cytochrome caa3 is a cytochrome c oxidase, whereas cytochrome bd and cytochrome aa3 are quinol oxidases. A fourth terminal oxidase, YthAB, is a putative quinol oxidase predicted from DNA sequence analysis. None of the terminal oxidases are, by themselves, essential for growth. However, one quinol oxidase (cytochrome aa3 or cytochrome bd) is required for aerobic growth of B. subtilis strain 168. Data indicating that cytochrome aa3 is the major oxidase used by exponentially growing cells in minimal and rich medium are presented. We show that one of the two heme-copper oxidases, cytochrome caa3 or cytochrome aa3, is required for efficient sporulation of B. subtilis strain 168 and that deletion of YthAB in a strain lacking cytochrome aa3 makes the strain sporulation deficient.

* Corresponding author. Mailing address: Lund University, Department of Microbiology, Sölvegatan 12, SE-223 62 Lund, Sweden. Phone: 46 46 222 3456. Fax: 46 46 157839. E-mail: claes.von_wachenfeldt{at}mikrbiol.lu.se.

Journal of Bacteriology, December 2000, p. 6557-6564, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brochier-Armanet, C., Talla, E., Gribaldo, S. (2009). The Multiple Evolutionary Histories of Dioxygen Reductases: Implications for the Origin and Evolution of Aerobic Respiration. Mol Biol Evol 26: 285-297 [Abstract] [Full Text]  
  • Hu, Y., Raengpradub, S., Schwab, U., Loss, C., Orsi, R. H., Wiedmann, M., Boor, K. J. (2007). Phenotypic and Transcriptomic Analyses Demonstrate Interactions between the Transcriptional Regulators CtsR and Sigma B in Listeria monocytogenes. Appl. Environ. Microbiol. 73: 7967-7980 [Abstract] [Full Text]  
  • Brooijmans, R. J. W., Poolman, B., Schuurman-Wolters, G. K., de Vos, W. M., Hugenholtz, J. (2007). Generation of a Membrane Potential by Lactococcus lactis through Aerobic Electron Transport. J. Bacteriol. 189: 5203-5209 [Abstract] [Full Text]  
  • Day, W. A. Jr., Rasmussen, S. L., Carpenter, B. M., Peterson, S. N., Friedlander, A. M. (2007). Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis: Global Identification of Genes Required for Sporulation and Germination. J. Bacteriol. 189: 3296-3301 [Abstract] [Full Text]  
  • Hederstedt, L., Lewin, A., Throne-Holst, M. (2005). Heme A Synthase Enzyme Functions Dissected by Mutagenesis of Bacillus subtilis CtaA. J. Bacteriol. 187: 8361-8369 [Abstract] [Full Text]  
  • Larsson, J. T., Rogstam, A., von Wachenfeldt, C. (2005). Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis. Microbiology 151: 3323-3335 [Abstract] [Full Text]  
  • Schau, M., Eldakak, A., Hulett, F. M. (2004). Terminal Oxidases Are Essential To Bypass the Requirement for ResD for Full Pho Induction in Bacillus subtilis. J. Bacteriol. 186: 8424-8432 [Abstract] [Full Text]  
  • Schau, M., Chen, Y., Hulett, F. M. (2004). Bacillus subtilis YdiH Is a Direct Negative Regulator of the cydABCD Operon. J. Bacteriol. 186: 4585-4595 [Abstract] [Full Text]  
  • Bengtsson, J., von Wachenfeldt, C., Winstedt, L., Nygaard, P., Hederstedt, L. (2004). CtaG is required for formation of active cytochrome c oxidase in Bacillus subtilis. Microbiology 150: 415-425 [Abstract] [Full Text]  
  • Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerich, S., Bessieres, P., Boland, F., Brignell, S. C., Bron, S., Bunai, K., Chapuis, J., Christiansen, L. C., Danchin, A., Debarbouille, M., Dervyn, E., Deuerling, E., Devine, K., Devine, S. K., Dreesen, O., Errington, J., Fillinger, S., Foster, S. J., Fujita, Y., Galizzi, A., Gardan, R., Eschevins, C., Fukushima, T., Haga, K., Harwood, C. R., Hecker, M., Hosoya, D., Hullo, M. F., Kakeshita, H., Karamata, D., Kasahara, Y., Kawamura, F., Koga, K., Koski, P., Kuwana, R., Imamura, D., Ishimaru, M., Ishikawa, S., Ishio, I., Le Coq, D., Masson, A., Mauel, C., Meima, R., Mellado, R. P., Moir, A., Moriya, S., Nagakawa, E., Nanamiya, H., Nakai, S., Nygaard, P., Ogura, M., Ohanan, T., O'Reilly, M., O'Rourke, M., Pragai, Z., Pooley, H. M., Rapoport, G., Rawlins, J. P., Rivas, L. A., Rivolta, C., Sadaie, A., Sadaie, Y., Sarvas, M., Sato, T., Saxild, H. H., Scanlan, E., Schumann, W., Seegers, J. F. M. L., Sekiguchi, J., Sekowska, A., Seror, S. J., Simon, M., Stragier, P., Studer, R., Takamatsu, H., Tanaka, T., Takeuchi, M., Thomaides, H. B., Vagner, V., van Dijl, J. M., Watabe, K., Wipat, A., Yamamoto, H., Yamamoto, M., Yamamoto, Y., Yamane, K., Yata, K., Yoshida, K., Yoshikawa, H., Zuber, U., Ogasawara, N. (2003). Essential Bacillussubtilis genes. Proc. Natl. Acad. Sci. USA 100: 4678-4683 [Abstract] [Full Text]  
  • Kana, B. D., Weinstein, E. A., Avarbock, D., Dawes, S. S., Rubin, H., Mizrahi, V. (2001). Characterization of the cydAB-Encoded Cytochrome bd Oxidase from Mycobacterium smegmatis. J. Bacteriol. 183: 7076-7086 [Abstract] [Full Text]  
  • Dauner, M., Storni, T., Sauer, U. (2001). Bacillus subtilis Metabolism and Energetics in Carbon-Limited and Excess-Carbon Chemostat Culture. J. Bacteriol. 183: 7308-7317 [Abstract] [Full Text]  
  • Paul, S., Zhang, X., Hulett, F. M. (2001). Two ResD-Controlled Promoters Regulate ctaA Expression in Bacillus subtilis. J. Bacteriol. 183: 3237-3246 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»