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Journal of Bacteriology, December 2000, p. 6584-6591, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Level of DAL80 Expression Down-Regulates GATA Factor-Mediated Transcription in Saccharomyces cerevisiae

Thomas S. Cunningham, Rajendra Rai, and Terrance G. Cooper*

Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 38163

Received 22 June 2000/Accepted 13 September 2000

Nitrogen-catabolic gene expression in Saccharomyces cerevisiae is regulated by the action of four GATA family transcription factors: Gln3p and Gat1p/Nil1p are transcriptional activators, and Dal80 and Deh1p/Gzf3p are repressors. In addition to the GATA sequences situated upstream of all nitrogen catabolite repression-sensitive genes that encode enzyme and transport proteins, the promoters of the GAT1, DAL80, and DEH1 genes all contain multiple GATA sequences as well. These GATA sequences are the binding sites of the GATA family transcription factors and are hypothesized to mediate their autogenous and cross regulation. Here we show, using DAL80 fused to the carbon-regulated GAL1,10 or copper-regulated CUP1 promoter, that GAT1 expression is inversely regulated by the level of DAL80 expression, i.e., as DAL80 expression increases, GAT1 expression decreases. The amount of DAL80 expression also dictates the level at which DAL3, a gene activated almost exclusively by Gln3p, is transcribed. Gat1p was found to partially substitute for Gln3p in transcription. These data support the contention that regulation of GATA-factor gene expression is tightly and dynamically coupled. Finally, we suggest that the complicated regulatory circuit in which the GATA family transcription factors participate is probably most beneficial as cells make the transition from excess to limited nitrogen availability.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Tennessee, Memphis, TN 38163. Phone: (901) 448-6175. Fax: (901) 448-8462. E-mail: tcooper{at}utmem.edu.

Journal of Bacteriology, December 2000, p. 6584-6591, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Georis, I., Feller, A., Vierendeels, F., Dubois, E. (2009). The Yeast GATA Factor Gat1 Occupies a Central Position in Nitrogen Catabolite Repression-Sensitive Gene Activation. Mol. Cell. Biol. 29: 3803-3815 [Abstract] [Full Text]  
  • Georis, I., Feller, A., Tate, J. J., Cooper, T. G., Dubois, E. (2009). Nitrogen Catabolite Repression-Sensitive Transcription as a Readout of Tor Pathway Regulation: The Genetic Background, Reporter Gene and GATA Factor Assayed Determine the Outcomes. Genetics 181: 861-874 [Abstract] [Full Text]  
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