This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Welch, T. J.
Right arrow Articles by Crosa, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welch, T. J.
Right arrow Articles by Crosa, J. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2000, p. 6762-6773, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Overlapping angB and angG Genes Are Encoded within the trans-Acting Factor Region of the Virulence Plasmid in Vibrio anguillarum: Essential Role in Siderophore Biosynthesis

Timothy J. Welch, Sunghee Chai, and Jorge H. Crosa*

Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201-3098

Received 18 April 2000/Accepted 24 July 2000

Products encoded in the trans-acting factor (TAF) region are necessary for the biosynthesis of anguibactin and for maximal expression of iron transport and biosynthesis genes in the plasmid-encoded iron-scavenging system of Vibrio anguillarum. Here we identify angB, a locus located in the TAF region, which encodes products essential for anguibactin biosynthesis. We demonstrate that a 287-amino-acid polypeptide, encoded by angB and designated AngB, has an isochorismate lyase activity necessary for the synthesis of 2,3-dihydroxybenzoic acid, an anguibactin biosynthesis intermediate. Complementation of various angB mutations provided evidence that an additional, overlapping gene exists at this locus. This second gene, designated angG, also has an essential biosynthetic function. The angG gene directs the expression of three polypeptides when overexpressed in Escherichia coli, all of which are translated in the same frame as AngB. The results of site-directed mutagenesis and in vivo phosphorylation experiments suggest that the carboxy-terminal end of AngB and the AngG polypeptide(s) function as aryl carrier proteins involved in the assembly of the anguibactin molecule. Our results also show that the regulatory functions of the TAF are encoded in a region, TAFr, which is distinct from and independent of the angB and angG genes.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, 3181 Sam Jackson Park Rd., Portland, OR 97201-3098. Phone: (503) 494-7583. Fax: (503) 494-6862. E-mail: crosajor{at}ohsu.edu.

Journal of Bacteriology, December 2000, p. 6762-6773, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Balado, M., Osorio, C. R., Lemos, M. L. (2008). Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum. Microbiology 154: 1400-1413 [Abstract] [Full Text]  
  • Stork, M., Otto, B. R., Crosa, J. H. (2007). A Novel Protein, TtpC, Is a Required Component of the TonB2 Complex for Specific Iron Transport in the Pathogens Vibrio anguillarum and Vibrio cholerae. J. Bacteriol. 189: 1803-1815 [Abstract] [Full Text]  
  • Welch, T. J., Crosa, J. H. (2005). Novel Role of the Lipopolysaccharide O1 Side Chain in Ferric Siderophore Transport and Virulence of Vibrio anguillarum. Infect. Immun. 73: 5864-5872 [Abstract] [Full Text]  
  • Alice, A. F., Lopez, C. S., Crosa, J. H. (2005). Plasmid- and Chromosome-Encoded Redundant and Specific Functions Are Involved in Biosynthesis of the Siderophore Anguibactin in Vibrio anguillarum 775: a Case of Chance and Necessity?. J. Bacteriol. 187: 2209-2214 [Abstract] [Full Text]  
  • Stork, M., Di Lorenzo, M., Mourino, S., Osorio, C. R., Lemos, M. L., Crosa, J. H. (2004). Two tonB Systems Function in Iron Transport in Vibrio anguillarum, but Only One Is Essential for Virulence. Infect. Immun. 72: 7326-7329 [Abstract] [Full Text]  
  • Di Lorenzo, M., Poppelaars, S., Stork, M., Nagasawa, M., Tolmasky, M. E., Crosa, J. H. (2004). A Nonribosomal Peptide Synthetase with a Novel Domain Organization Is Essential for Siderophore Biosynthesis in Vibrio anguillarum. J. Bacteriol. 186: 7327-7336 [Abstract] [Full Text]  
  • Tanabe, T., Funahashi, T., Nakao, H., Miyoshi, S.-I., Shinoda, S., Yamamoto, S. (2003). Identification and Characterization of Genes Required for Biosynthesis and Transport of the Siderophore Vibrioferrin in Vibrio parahaemolyticus. J. Bacteriol. 185: 6938-6949 [Abstract] [Full Text]  
  • Di Lorenzo, M., Stork, M., Tolmasky, M. E., Actis, L. A., Farrell, D., Welch, T. J., Crosa, L. M., Wertheimer, A. M., Chen, Q., Salinas, P., Waldbeser, L., Crosa, J. H. (2003). Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish Pathogen Vibrio anguillarum Strain 775. J. Bacteriol. 185: 5822-5830 [Abstract] [Full Text]  
  • Dorsey, C. W., Tolmasky, M. E., Crosa, J. H., Actis, L. A. (2003). Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transport. Microbiology 149: 1227-1238 [Abstract] [Full Text]  
  • Holmstrom, K., Gram, L. (2003). Elucidation of the Vibrio anguillarum Genetic Response to the Potential Fish Probiont Pseudomonas fluorescens AH2, Using RNA-Arbitrarily Primed PCR. J. Bacteriol. 185: 831-842 [Abstract] [Full Text]  
  • Crosa, J. H., Walsh, C. T. (2002). Genetics and Assembly Line Enzymology of Siderophore Biosynthesis in Bacteria. Microbiol. Mol. Biol. Rev. 66: 223-249 [Abstract] [Full Text]  
  • Lynch, D., O'Brien, J., Welch, T., Clarke, P., Ó Cuív, P., Crosa, J. H., O'Connell, M. (2001). Genetic Organization of the Region Encoding Regulation, Biosynthesis, and Transport of Rhizobactin 1021, a Siderophore Produced by Sinorhizobium meliloti. J. Bacteriol. 183: 2576-2585 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»