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Journal of Bacteriology, December 2000, p. 6819-6823, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

YsxC, a Putative GTP-Binding Protein Essential for Growth of Bacillus subtilis 168

Zoltán Prágai and Colin R. Harwood*

Department of Microbiology and Immunology, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom

Received 19 July 2000/Accepted 6 September 2000

YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is essential for growth, we attempted to construct mutants in which ysxC was either inactivated or placed under the control of an inducible promoter. Viable mutants were obtained only in the latter case, and these were inducer dependent, demonstrating unambiguously that ysxC is an essential gene.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The Medical School, University of Newcastle upon Tyne, Framlington Pl., Newcastle upon Tyne, NE2 4HH, United Kingdom. Phone: 44 191 222 7708. Fax: 44 191 222 7736. E-mail: Colin.Harwood{at}ncl.ac.uk.

Journal of Bacteriology, December 2000, p. 6819-6823, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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