This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stedman, K. M.
Right arrow Articles by Zillig, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stedman, K. M.
Right arrow Articles by Zillig, W.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2000, p. 7014-7020, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

pING Family of Conjugative Plasmids from the Extremely Thermophilic Archaeon Sulfolobus islandicus: Insights into Recombination and Conjugation in Crenarchaeota

Kenneth M. Stedman,1,dagger Qunxin She,2 Hien Phan,2 Ingelore Holz,1 Harpreet Singh,1 David Prangishvili,1,Dagger Roger Garrett,2 and Wolfram Zillig1,*

Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany,1 and Institute of Molecular Biology, University of Copenhagen K, Sølvgade 83H, DK-1307 Copenhagen K, Denmark2

Received 17 April 2000/Accepted 21 September 2000

A novel family of conjugative plasmids from Sulfolobus comprising the active variants pING1, -4, and -6 and the functionally defective variants pING2 and -3, which require the help of an active variant for spreading, has been extensively characterized both functionally and molecularly. In view of the sparse similarity between bacterial and archaeal conjugation and the lack of a practical genetic system for Sulfolobus, we compared the functions and sequences of these variants and the previously described archaeal conjugative plasmid pNOB8 in order to identify open reading frames (ORFs) and DNA sequences that are involved in conjugative transfer and maintenance of these plasmids in Sulfolobus. The variants pING4 and -6 are reproducibly derived from pING1 in vivo by successive transpositions of an element from the Sulfolobus genome. The small defective but mobile variants pING2 and -3, which both lack a cluster of highly conserved ORFs probably involved in plasmid transfer, were shown to be formed in vivo by recombinative deletion of the larger part of the genomes of pING4 and pING6, respectively. The efficient occurrence of these recombination processes is further evidence for the striking plasticity of the Sulfolobus genome.

* Corresponding author. Mailing address: Max Planck Institute for Biochemistry, Am Klopferspitz 18A, D-82152 Martinsried, Germany. Phone: 49-89-8578-2231. Fax: 49-89-8578-2728. E-mail: zillig{at}biochem.mpg.de.

dagger Present address: Thermal Biology Institute, Montana State University, Bozeman, MT 59717-3142.

Dagger Present address: Department of Microbiology, University of Regensburg, 95053 Regensburg, Germany.

Journal of Bacteriology, December 2000, p. 7014-7020, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zaneveld, J. R., Nemergut, D. R., Knight, R. (2008). Are all horizontal gene transfers created equal? Prospects for mechanism-based studies of HGT patterns. Microbiology 154: 1-15 [Abstract] [Full Text]  
  • Aucelli, T., Contursi, P., Girfoglio, M., Rossi, M., Cannio, R. (2006). A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus. Nucleic Acids Res 34: e114-e114 [Abstract] [Full Text]  
  • Erauso, G., Stedman, K. M., van de Werken, H. J. G., Zillig, W., van der Oost, J. (2006). Two novel conjugative plasmids from a single strain of Sulfolobus. Microbiology 152: 1951-1968 [Abstract] [Full Text]  
  • Bartolucci, S., Rossi, M., Cannio, R. (2003). Characterization and Functional Complementation of a Nonlethal Deletion in the Chromosome of a {beta}-Glycosidase Mutant of Sulfolobus solfataricus. J. Bacteriol. 185: 3948-3957 [Abstract] [Full Text]  
  • Lipps, G., Ibanez, P., Stroessenreuther, T., Hekimian, K., Krauss, G. (2001). The protein ORF80 from the acidophilic and thermophilic archaeon Sulfolobus islandicus binds highly site-specifically to double-stranded DNA and represents a novel type of basic leucine zipper protein. Nucleic Acids Res 29: 4973-4982 [Abstract] [Full Text]  
  • She, Q., Singh, R. K., Confalonieri, F., Zivanovic, Y., Allard, G., Awayez, M. J., Chan-Weiher, C. C.-Y., Clausen, I. G., Curtis, B. A., De Moors, A., Erauso, G., Fletcher, C., Gordon, P. M. K., Heikamp-de Jong, I., Jeffries, A. C., Kozera, C. J., Medina, N., Peng, X., Thi-Ngoc, H. P., Redder, P., Schenk, M. E., Theriault, C., Tolstrup, N., Charlebois, R. L., Doolittle, W. F., Duguet, M., Gaasterland, T., Garrett, R. A., Ragan, M. A., Sensen, C. W., Van der Oost, J. (2001). The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl. Acad. Sci. USA 10.1073/pnas.141222098v1 [Abstract] [Full Text]  
  • She, Q., Singh, R. K., Confalonieri, F., Zivanovic, Y., Allard, G., Awayez, M. J., Chan-Weiher, C. C.-Y., Clausen, I. G., Curtis, B. A., De Moors, A., Erauso, G., Fletcher, C., Gordon, P. M. K., Heikamp-de Jong, I., Jeffries, A. C., Kozera, C. J., Medina, N., Peng, X., Thi-Ngoc, H. P., Redder, P., Schenk, M. E., Theriault, C., Tolstrup, N., Charlebois, R. L., Doolittle, W. F., Duguet, M., Gaasterland, T., Garrett, R. A., Ragan, M. A., Sensen, C. W., Van der Oost, J. (2001). The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl. Acad. Sci. USA 98: 7835-7840 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»