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Journal of Bacteriology, February 2000, p. 771-781, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2

Anthea K. Lee,* Corrella S. Detweiler, and Stanley Falkow

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305

Received 23 August 1999/Accepted 1 November 1999

Salmonella pathogenicity island 2 (SPI-2) encodes a putative, two-component regulatory system, SsrA-SsrB, which regulates a type III secretion system needed for replication inside macrophages and systemic infection in mice. The sensor and regulator homologs, ssrAB (spiR), and genes within the secretion system, including the structural gene ssaH, are transcribed after Salmonella enters host cells. We have studied the transcriptional regulation of ssrAB and the secretion system by using gfp fusions to the ssrA and ssaH promoters. We found that early transcription of ssrA, after entry into macrophages, is most efficient in the presence of OmpR. An ompR mutant strain does not exhibit replication within cultured macrophages. Furthermore, footprint analysis shows that purified OmpR protein binds directly to the ssrA promoter region. We also show that minimal medium, pH 4.5, induces SPI-2 gene expression in wild-type but not ompR mutant strains. We conclude that the type III secretion system of SPI-2 is regulated by OmpR, which activates expression of ssrA soon after Salmonella enters the macrophage.

* Corresponding author. Mailing address: Dept. Microbiology and Immunology, Stanford University School of Medicine, 299 Campus Dr., D300, Stanford, CA 94305-5124. Phone: (650) 723-2671. Fax: (650) 723-1837. E-mail: aklee{at}leland.stanford.edu.

Journal of Bacteriology, February 2000, p. 771-781, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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