This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ray, M.-C.
Right arrow Articles by Lazzaroni, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ray, M.-C.
Right arrow Articles by Lazzaroni, J. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2000, p. 821-824, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification by Genetic Suppression of Escherichia coli TolB Residues Important for TolB-Pal Interaction

Marie-Céline Ray, Pierre Germon, Anne Vianney, Raymond Portalier, and Jean Claude Lazzaroni*

Unité de Microbiologie et Génétique, UMR 5577, CNRS-INSA-Université Lyon I, F-69622 Villeurbanne, France

Received 12 July 1999/Accepted 8 November 1999

The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filamentous bacteriophages. TolQ, TolR, and TolA are located in the cytoplasmic membrane where they interact with each other via their transmembrane domains. TolB and Pal form a periplasmic complex near the outer membrane. We used suppressor genetics to identify the regions important for the interaction between TolB and Pal. Intragenic suppressor mutations were characterized in a domain of Pal that was shown to be involved in interactions with TolB and peptidoglycan. Extragenic suppressor mutations were located in tolB gene. The C-terminal region of TolB predicted to adopt a beta -propeller structure was shown to be responsible for the interaction of the protein with Pal. Unexpectedly, none of the suppressor mutations was able to restore a correct association between Pal and peptidoglycan, suggesting that interactions between Pal and other components such as TolB may also be important for outer membrane stability.

* Corresponding author. Mailing address: Unité de Microbiologie et Génétique, UMR 5577, bât 405, F-69622 Villeurbanne Cedex, France. Phone: (33) 472 43 13 67. Fax: (33) 472 43 26 86. E-mail: lazzaroni{at}biomserv.univ-lyon1.fr.

Journal of Bacteriology, February 2000, p. 821-824, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Merritt, M. E., Donaldson, J. R. (2009). Effect of bile salts on the DNA and membrane integrity of enteric bacteria. J Med Microbiol 58: 1533-1541 [Abstract] [Full Text]  
  • Li, L., Liang, S., Pilcher, M. M., Meroueh, S. O. (2009). Incorporating receptor flexibility in the molecular design of protein interfaces. Protein Eng Des Sel 22: 575-586 [Abstract] [Full Text]  
  • Hizukuri, Y., Morton, J. F., Yakushi, T., Kojima, S., Homma, M. (2009). The peptidoglycan-binding (PGB) Domain of the Escherichia coli Pal Protein can also Function as the PGB Domain in E. coli Flagellar Motor Protein MotB. J Biochem 146: 219-229 [Abstract] [Full Text]  
  • Hinchliffe, S. J., Howard, S. L., Huang, Y. H., Clarke, D. J., Wren, B. W. (2008). The importance of the Rcs phosphorelay in the survival and pathogenesis of the enteropathogenic yersiniae. Microbiology 154: 1117-1131 [Abstract] [Full Text]  
  • Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
  • Loftus, S. R., Walker, D., Mate, M. J., Bonsor, D. A., James, R., Moore, G. R., Kleanthous, C. (2006). Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9. Proc. Natl. Acad. Sci. USA 103: 12353-12358 [Abstract] [Full Text]  
  • Dubuisson, J.-F., Vianney, A., Hugouvieux-Cotte-Pattat, N., Lazzaroni, J. C. (2005). Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence. Microbiology 151: 3337-3347 [Abstract] [Full Text]  
  • Llamas, M. A., Rodriguez-Herva, J. J., Hancock, R. E. W., Bitter, W., Tommassen, J., Ramos, J. L. (2003). Role of Pseudomonas putida tol-oprL Gene Products in Uptake of Solutes through the Cytoplasmic Membrane. J. Bacteriol. 185: 4707-4716 [Abstract] [Full Text]  
  • Tamayo, R., Ryan, S. S., McCoy, A. J., Gunn, J. S. (2002). Identification and Genetic Characterization of PmrA-Regulated Genes and Genes Involved in Polymyxin B Resistance in Salmonella enterica Serovar Typhimurium. Infect. Immun. 70: 6770-6778 [Abstract] [Full Text]  
  • Dubuisson, J. F., Vianney, A., Lazzaroni, J. C. (2002). Mutational Analysis of the TolA C-Terminal Domain of Escherichia coli and Genetic Evidence for an Interaction between TolA and TolB. J. Bacteriol. 184: 4620-4625 [Abstract] [Full Text]  
  • Cascales, E., Bernadac, A., Gavioli, M., Lazzaroni, J.-C., Lloubes, R. (2002). Pal Lipoprotein of Escherichia coli Plays a Major Role in Outer Membrane Integrity. J. Bacteriol. 184: 754-759 [Abstract] [Full Text]  
  • Germon, P., Ray, M.-C., Vianney, A., Lazzaroni, J. C. (2001). Energy-Dependent Conformational Change in the TolA Protein of Escherichia coli Involves Its N-Terminal Domain, TolQ, and TolR. J. Bacteriol. 183: 4110-4114 [Abstract] [Full Text]  
  • Fortney, K. R., Young, R. S., Bauer, M. E., Katz, B. P., Hood, A. F., Munson, R. S. Jr., Spinola, S. M. (2000). Expression of Peptidoglycan-Associated Lipoprotein Is Required for Virulence in the Human Model of Haemophilus ducreyi Infection. Infect. Immun. 68: 6441-6448 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»