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Journal of Bacteriology, February 2000, p. 1109-1117, Vol. 182, No. 4
Salmonella Genetic Stock Centre, Department
of Biological Sciences, University of Calgary, Calgary, Alberta,
Canada T2N 1N4
Received 13 August 1999/Accepted 17 November 1999
Intervening sequences (IVSs) were originally identified in the
rrl genes for 23S rRNA (rrl genes, for large
ribosomal subunit, part of rrn operon encoding rRNA) of
Salmonella enterica serovars Typhimurium LT2 and Arizonae.
These sequences are transcribed but later removed during RNase III
processing of the rRNA, resulting in fragmentation of the 23S species;
IVSs are uncommon, but have been reported in at least 10 bacterial
genera. Through PCR amplification of IVS-containing regions of the
rrl genes we showed that most Proteus and
Providencia strains contain IVSs similar to those of
serovar Typhimurium in distribution and location in rrl
genes. By extraction and Northern blotting of rRNA, we also found that these IVSs result in rRNA fragmentation. We report the first finding of
two very different sizes of IVS (113 bp and 183 to 187 bp) in different
rrl genes in the same strain, in helix 25 of
Proteus and Providencia spp.; IVSs from helix
45 are 113 to 123 bp in size. Analysis of IVS sequence and postulated
secondary structure reveals striking similarities of
Proteus and Providencia IVSs to those of
serovar Typhimurium, with the stems of the smaller IVSs from helix 25 being similar to those of Salmonella helix 25 IVSs and with
both the stem and the central loop domain of helix 45 IVSs being
similar. Thus, IVSs of related sequences are widely distributed
throughout the Enterobacteriaceae, in
Salmonella, Yersinia, Proteus, and
Providencia spp., but we did not find them in
Escherichia coli, Citrobacter,
Enterobacter, Klebsiella, or Morganella spp.; the sporadic distribution of IVSs of
related sequence indicates that lateral genetic transfer has occurred.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Fragmentation of 23S rRNA in Strains of Proteus and
Providencia Results from Intervening Sequences in the
rrn (rRNA) Genes
*
Corresponding author. Mailing address: Department of
Biological Sciences, University of Calgary, 2500 University Dr., NW, Calgary, Alberta T2N 1N4, Canada. Phone: (403) 220-6792. Fax: (403)
289-9311. E-mail: kesander{at}ucalgary.ca.
Present address: Department of Microbiology, University of Guelph,
Guelph, Ontario, Canada N1G 2W1.
Journal of Bacteriology, February 2000, p. 1109-1117, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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