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Journal of Bacteriology, February 2000, p. 1144-1149, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa

Kathryn L. Hester,1 Jodi Lehman,1 Fares Najar,2 Lin Song,2 Bruce A. Roe,2 Carolyn H. MacGregor,3 Paul W. Hager,3 Paul V. Phibbs Jr.,3 and John R. Sokatch1,4,*

Department of Biochemistry and Molecular Biology1 and Department of Microbiology and Immunology,4 University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190; University of Oklahoma Advanced Center for Genomic Technology, Norman, Oklahoma2; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 278583

Received 21 May 1999/Accepted 11 November 1999

Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of the bkd operon, were isolated and identified as crc and vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190. Phone: (405) 271-2227. Fax: (405) 271-3092. E-mail: john-sokatch{at}ouhsc.edu.

Journal of Bacteriology, February 2000, p. 1144-1149, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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