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Journal of Bacteriology, February 2000, p. 1150-1153, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Catabolite Repression Control by Crc in 2xYT Medium Is Mediated by Posttranscriptional Regulation of bkdR Expression in Pseudomonas putida

Kathryn L. Hester,1 K. T. Madhusudhan,1 and John R. Sokatch1,2,*

Department of Biochemistry and Molecular Biology1 and Department of Microbiology and Immunology,2 University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190

Received 21 May 1999/Accepted 11 November 1999

The effect of growth in 2xYT medium on catabolite repression control in Pseudomonas putida has been investigated using the bkd operon, encoding branched-chain keto acid dehydrogenase. Crc (catabolite repression control protein) was shown to be responsible for repression of bkd operon transcription in 2xYT. BkdR levels were elevated in a P. putida crc mutant, but bkdR transcript levels were the same in both wild type and crc mutant. This suggests that the mechanism of catabolite repression control in rich media by Crc involves posttranscriptional regulation of the bkdR message.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190. Phone: (405) 271-2227. Fax: (405) 271-3091. E-mail: john-sokatch{at}ouhsc.edu.

Journal of Bacteriology, February 2000, p. 1150-1153, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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