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Journal of Bacteriology, February 2000, p. 1176-1180, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systemsdagger

Susan Kinder Haake,1,2,* Sean C. Yoder,1 Gwynne Attarian,1 and Kara Podkaminer1

Divisions of Associated Clinical Sciences1 and Oral Biology and Medicine,2 UCLA School of Dentistry, Los Angeles, California

Received 15 July 1999/Accepted 19 November 1999

Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

* Corresponding author. Mailing address: Section of Periodontics, UCLA School of Dentistry, 10833 Le Conte Ave., Los Angeles, CA 90095-1668. Phone: (310) 794-7163. Fax: (310) 206-3282. E-mail: shaake{at}dent.ucla.edu.

dagger We dedicate this work to the memory of Susan E. Valone in recognition of her support and contributions to this research.

Journal of Bacteriology, February 2000, p. 1176-1180, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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