This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Claus, H.
Right arrow Articles by Vogel, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Claus, H.
Right arrow Articles by Vogel, U.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2000, p. 1296-1303, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differential Distribution of Novel Restriction-Modification Systems in Clonal Lineages of Neisseria meningitidis

Heike Claus, Alexander Friedrich, Matthias Frosch, and Ulrich Vogel*

Institut für Hygiene und Mikrobiologie, University of Würzburg, Würzburg, Germany

Received 3 September 1999/Accepted 30 November 1999

Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster A4 meningococci. NmeDI was harbored by meningococci of the ET-37 complex and of cluster A4, but not by serogroup A meningococci. Two of the R-M systems, NmeBI and NmeDI, were located at homologous positions between the phenylalanyl-tRNA synthetase genes pheS and pheT, which appeared to be a preferential target for the insertion of foreign DNA in meningococci. The distribution of the three R-M systems was tested with 103 meningococcal strains comprising 49 sequence types. The vast majority of the strains had either NmeBI, NmeAI, or both NmeAI and NmeDI. Using cocultivation experiments, we could demonstrate that NmeBI, which was present in ET-5 complex meningococci, was responsible for a partial restriction of DNA transfer from meningococci of the ET-37 complex to meningococci of the ET-5 complex.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie, Universität Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany. Phone: 49(931)201 3902. Fax: 49(931)201 3445. E-mail: uvogel{at}hygiene.uni-wuerzburg.de.

Journal of Bacteriology, March 2000, p. 1296-1303, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Snyder, L. A. S., McGowan, S., Rogers, M., Duro, E., O'Farrell, E., Saunders, N. J. (2007). The Repertoire of Minimal Mobile Elements in the Neisseria Species and Evidence That These Are Involved in Horizontal Gene Transfer in Other Bacteria. Mol Biol Evol 24: 2802-2815 [Abstract] [Full Text]  
  • Hotopp, J. C. D., Grifantini, R., Kumar, N., Tzeng, Y. L., Fouts, D., Frigimelica, E., Draghi, M., Giuliani, M. M., Rappuoli, R., Stephens, D. S., Grandi, G., Tettelin, H. (2006). Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen-specific genes. Microbiology 152: 3733-3749 [Abstract] [Full Text]  
  • Callaghan, M. J., Jolley, K. A., Maiden, M. C. J. (2006). Opacity-Associated Adhesin Repertoire in Hyperinvasive Neisseria meningitidis. Infect. Immun. 74: 5085-5094 [Abstract] [Full Text]  
  • Kwiatek, A., Kobes, M., Olejnik, K., Piekarowicz, A. (2004). DNA methyltransferases from Neisseria meningitidis and Neisseria gonorrhoeae FA1090 associated with mismatch nicking endonucleases. Microbiology 150: 1713-1722 [Abstract] [Full Text]  
  • Claus, H., Weinand, H., Frosch, M., Vogel, U. (2003). Identification of the Hypervirulent Lineages of Neisseria meningitidis, the ST-8 and ST-11 Complexes, by Using Monoclonal Antibodies Specific to NmeDI. J. Clin. Microbiol. 41: 3873-3876 [Abstract] [Full Text]  
  • Chanto, G., Occhialini, A., Gras, N., Alm, R. A., Megraud, F., Marais, A. (2002). Identification of strain-specific genes located outside the plasticity zone in nine clinical isolates of Helicobacter pylori. Microbiology 148: 3671-3680 [Abstract] [Full Text]  
  • Claus, H., Maiden, M. C. J., Maag, R., Frosch, M., Vogel, U. (2002). Many carried meningococci lack the genes required for capsule synthesis and transport. Microbiology 148: 1813-1819 [Abstract] [Full Text]  
  • Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y. (2001). Distribution of the SsuDAT1I Restriction-Modification System among Different Serotypes of Streptococcus suis. J. Bacteriol. 183: 5436-5440 [Abstract] [Full Text]  
  • Kobayashi, I. (2001). Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution. Nucleic Acids Res 29: 3742-3756 [Abstract] [Full Text]  
  • Alber, D., Oberkötter, M., Suerbaum, S., Claus, H., Frosch, M., Vogel, U. (2001). Genetic Diversity of Neisseria lactamica Strains from Epidemiologically Defined Carriers. J. Clin. Microbiol. 39: 1710-1715 [Abstract] [Full Text]  
  • Claus, H., Stoevesandt, J., Frosch, M., Vogel, U. (2001). Genetic Isolation of Meningococci of the Electrophoretic Type 37 Complex. J. Bacteriol. 183: 2570-2575 [Abstract] [Full Text]  
  • Bart, A., Pannekoek, Y., Dankert, J., van der Ende, A. (2001). NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain. Infect. Immun. 69: 1816-1820 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»