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Journal of Bacteriology, March 2000, p. 1415-1418, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Physical Map of the Chromosome of the Apple Proliferation Phytoplasma

Ulrich Lauer and Erich Seemüller*

Biologische Bundesanstalt für Land-und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, Dossenheim, Germany

Received 14 July 1999/Accepted 24 November 1999

A physical map of the apple proliferation phytoplasma strain AT chromosome was constructed from genomic DNA extracted from diseased tobacco plants. The map was generated with single and double digestions of the chromosome with BssHII, SmaI, MluI, and ApaI restriction endonucleases and resolving the fragments by pulsed-field gel electrophoresis. Partial digestion and Southern blot analysis were used to assist in the arrangement of the 14 contiguous restriction fragments obtained. From the restriction fragments generated by double digestions, the size of the circular chromosome was calculated to be approximately 645 kb. Locations of the two rRNA operons, the operon including the fus and tuf genes, and three other genes were placed on the map. Genome sizes and BssHII restriction profiles of apple proliferation strain AP15 and the pear decline and European stone fruit yellows phytoplasmas were different from that of strain AT.

* Corresponding author. Mailing address: Biologische Bundesanstalt für Land-und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, D-69221 Dossenheim, Germany. Phone: 49 6221 868 0550. Fax: 49 6221 868 0515. E-mail: erich.seemueller{at}urz.uni-heidelberg.de.

Journal of Bacteriology, March 2000, p. 1415-1418, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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