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Journal of Bacteriology, March 2000, p. 1442-1447, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytochrome c' from Rhodobacter capsulatus Confers Increased Resistance to Nitric Oxide

Richard Cross, Joanne Aish, Samantha J. Paston, Robert K. Poole, and James W. B. Moir*

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom

Received 14 September 1999/Accepted 5 December 1999

We report the cloning and sequencing of the gene containing cytochrome c' (cycP) from the photosynthetic purple bacterium Rhodobacter capsulatus and the regions flanking that gene. Mutant strains unable to synthesize cytochrome c' had increased sensitivity to nitrosothiols and to nitric oxide (which binds to the heme moiety of cytochrome c').


* Corresponding author. Mailing address: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44 (0) 114 2224409. Fax: 44 (0) 114 2728697. E-mail: j.moir{at}sheffield.ac.uk.


Journal of Bacteriology, March 2000, p. 1442-1447, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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