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Journal of Bacteriology, March 2000, p. 1442-1447, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Cytochrome c' from Rhodobacter
capsulatus Confers Increased Resistance to Nitric Oxide
Richard
Cross,
Joanne
Aish,
Samantha J.
Paston,
Robert
K.
Poole, and
James W. B.
Moir*
Department of Molecular Biology and
Biotechnology, University of Sheffield, Sheffield S10 2TN, United
Kingdom
Received 14 September 1999/Accepted 5 December 1999
We report the cloning and sequencing of the gene containing
cytochrome c' (cycP) from the photosynthetic
purple bacterium Rhodobacter capsulatus and the regions
flanking that gene. Mutant strains unable to synthesize cytochrome
c' had increased sensitivity to nitrosothiols and to nitric
oxide (which binds to the heme moiety of cytochrome c').
*
Corresponding author. Mailing address: Department of
Molecular Biology and Biotechnology, University of Sheffield, Firth
Court, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44 (0) 114 2224409. Fax: 44 (0) 114 2728697. E-mail:
j.moir{at}sheffield.ac.uk.
Journal of Bacteriology, March 2000, p. 1442-1447, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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