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Journal of Bacteriology, March 2000, p. 1448-1451, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Utilization of Subsidiary Chromosomal Replication Terminators in Bacillus subtilis

A. A. Griffiths and R. G. Wake*

Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia

Received 23 August 1999/Accepted 3 December 1999

The Bacillus subtilis merodiploid strain GSY1127 contains a large nontandem duplication of a portion of its chromosome within its left (anticlockwise) replication segment. This causes displacement of the replication terminus region to a noticeably asymmetric location relative to oriC. The utilization of the subsidiary replication terminators, TerIII and TerV, in the merodiploid strain has been compared with that in B. subtilis 168. It is shown that TerIII is utilized to a significant extent in GSY1127 and that TerV is used only marginally at the most. Neither of these terminators is used to a measurable extent in the 168 strain. It is concluded that TerIII and TerV do indeed function as backups to the major terminator TerI, as has been generally thought. It is further concluded that, in the 168 strain, the vast majority of clockwise forks are arrested at the highly efficient TerI terminator, with fork fusion between the approaching forks occurring frequently while the clockwise fork is stationary at TerI.

* Corresponding author. Mailing address: Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia. Phone: (61-2) 9351-2504. Fax: (61-2) 9251-4726. E-mail: G.Wake{at}biochem.usyd.edu.au.

Journal of Bacteriology, March 2000, p. 1448-1451, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Lemon, K. P., Kurtser, I., Grossman, A. D. (2000). Effects of replication termination mutants on chromosome partitioning in Bacillussubtilis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011506098v1 [Abstract] [Full Text]  
  • Lemon, K. P., Kurtser, I., Grossman, A. D. (2001). Effects of replication termination mutants on chromosome partitioning in Bacillussubtilis. Proc. Natl. Acad. Sci. USA 98: 212-217 [Abstract] [Full Text]  


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