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Journal of Bacteriology, April 2000, p. 1949-1955, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A 90-Kilobase Conjugative Chromosomal Element Coding for Biphenyl and Salicylate Catabolism in Pseudomonas putida KF715

Akito Nishi, Kiyomi Tominaga, and Kensuke Furukawa*

Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan

Received 4 November 1999/Accepted 30 December 1999

The biphenyl and salicylate metabolic pathways in Pseudomonas putida KF715 are chromosomally encoded. The bph gene cluster coding for the conversion of biphenyl to benzoic acid and the sal gene cluster coding for the salicylate meta-pathway were obtained from the KF715 genomic cosmid libraries. These two gene clusters were separated by 10-kb DNA and were highly prone to deletion when KF715 was grown in nutrient medium. Two types of deletions took place at the region including only the bph genes (ca. 40 kb) or at the region including both the bph and sal genes (ca. 70 kb). A 90-kb DNA region, including both the bph and sal genes (termed the bph-sal element), was transferred by conjugation from KF715 to P. putida AC30. Such transconjugants gained the ability to grow on biphenyl and salicylate as the sole sources of carbon. The bph and sal element was located on the chromosome of the recipient. The bph-sal element in strain AC30 was also highly prone to deletion; however, it could be mobilized to the chromosome of P. putida KT2440 and the two deletion mutants of KF715.

* Corresponding author. Mailing address: Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan. Phone: (92) 642-2849. Fax: (92) 642-2849. E-mail: kfurukaw{at}agr.kyushu-u.ac.jp.

Journal of Bacteriology, April 2000, p. 1949-1955, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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