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Journal of Bacteriology, April 2000, p. 1964-1968, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Repair of DNA Lesions Induced by Hydrogen Peroxide in the Presence of Iron Chelators in Escherichia coli: Participation of Endonuclease IV and Fpg

Rodrigo S. Galhardo, Carlos E. B. Almeida, Alvaro C. Leitão, and Januário B. Cabral-Neto*

Laboratório de Radiobiologia Molecular, Programa de Biologia Molecular, Instituto de Biofisica Carlos Chagas Filho, Centro de Ciências da Saúde-Bloco G, Universidade Federal do Rio de Janeiro, CEP 21949-900 Rio de Janeiro, RJ, Brazil

Received 30 August 1999/Accepted 12 January 2000

In Escherichia coli, the repair of lethal DNA damage induced by H2O2 requires exonuclease III, the xthA gene product. Here, we report that both endonuclease IV (the nfo gene product) and exonuclease III can mediate the repair of lesions induced by H2O2 under low-iron conditions. Neither the xthA nor the nfo mutants was sensitive to H2O2 in the presence of iron chelators, while the xthA nfo double mutant was significantly sensitive to this treatment, suggesting that both exonuclease III and endonuclease IV can mediate the repair of DNA lesions formed under such conditions. Sedimentation studies in alkaline sucrose gradients also demonstrated that both xthA and nfo mutants, but not the xthA nfo double mutant, can carry out complete repair of DNA strand breaks and alkali-labile bonds generated by H2O2 under low-iron conditions. We also found indications that the formation of substrates for exonuclease III and endonuclease IV is mediated by the Fpg DNA glycosylase, as suggested by experiments in which the fpg mutation increased the level of cell survival, as well as repair of DNA strand breaks, in an AP endonuclease-null background.

* Corresponding author. Mailing address: Laboratório de Radiobiologia Molecular, Programa de Biologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde-Bloco G, Universidade Federal do Rio de Janeiro, CEP 21949-900 Rio de Janeiro, RJ, Brazil. Phone: 55 21 590-7147. Fax: 55 21 280 8193. E-mail: cabral{at}biof.ufrj.br.

Journal of Bacteriology, April 2000, p. 1964-1968, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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