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Journal of Bacteriology, April 2000, p. 2329-2331, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Bacillus subtilis Gene of Previously Unknown Function, yhaG, Is Translationally Regulated by Tryptophan-Activated TRAP and Appears To Be Involved in Tryptophan Transport

Joseph P. Sarsero,1 Enrique Merino,2 and Charles Yanofsky1,*

Department of Biological Sciences, Stanford University, Stanford, California 94305-5020,1 and Department of Molecular Microbiology, Instituto de Biotecnología, Universidad Nacional Autonoma de México, Cuernavaca, Morelos 62271, Mexico2

Received 28 December 1999/Accepted 28 January 2000

Computer analysis of the Bacillus subtilis genome sequence revealed a gene with no previously attributed function, yhaG, specifying a transcript containing a presumptive binding site for the tryptophan-activated regulatory protein, TRAP. The presumptive TRAP binding site overlaps the yhaG Shine-Dalgarno sequence and translation initiation region. TRAP was shown to regulate expression of yhaG translationally. Production of the yhaG transcript in vivo was found to compete for the binding of TRAP to other known TRAP binding sites. YhaG is likely to be a transmembrane protein involved in tryptophan transport.

* Corresponding author. Mailing address: Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020. Phone: (650) 725-1835. Fax: (650) 725-8221. E-mail: yanofsky{at}cmgm.stanford.edu.

Journal of Bacteriology, April 2000, p. 2329-2331, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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