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Journal of Bacteriology, April 2000, p. 2350-2353, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of an Operon Required for Ferrichrome Iron Utilization in Vibrio cholerae

Marc B. Rogers,1,dagger Jessica A. Sexton,1,Dagger G. Joel DeCastro,1,§ and Stephen B. Calderwood1,2,*

Infectious Disease Division, Massachusetts General Hospital, Boston, Massachusetts 02114,1 and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021152

Received 15 November 1999/Accepted 24 January 2000

Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous to Escherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhu operon, the V. cholerae ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.

* Corresponding author. Mailing address: Infectious Disease Division, Jackson 504, Massachusetts General Hospital, 55 Fruit St., Boston, MA 02114. Phone: (617) 726-3811. Fax: (617) 726-7416. E-mail: scalderwood{at}partners.org.

dagger Present address: EcoSoil Systems, Inc., San Diego, CA 92127.

Dagger Present address: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.

§ Present address: College of Physicians and Surgeons, Columbia University, New York, NY 10032.

Journal of Bacteriology, April 2000, p. 2350-2353, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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