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Journal of Bacteriology, May 2000, p. 2591-2596, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mutation of ndh Genes Leads to Inhibition of CO2 Uptake Rather than HCO3minus Uptake in Synechocystis sp. Strain PCC 6803

Hiroshi Ohkawa,1 G. Dean Price,2 Murray R. Badger,2 and Teruo Ogawa1,*

Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan,1 and Molecular Plant Physiology Group, Research School of Biological Sciences, Australian National University, Canberra 2601, Australia2

Received 19 August 1999/Accepted 8 January 2000

Six mutants (B1 to B6) that grew poorly in air on BG11 agar plates buffered at pH 8.0 were rescued after mutations were introduced into ndhB of wild-type (WT) Synechocystis sp. strain PCC 6803. In these mutants and a mutant (M55) lacking ndhB, CO2 uptake was much more strongly inhibited than HCO3- uptake, i.e., the activities of CO2 and HCO3- uptake in B1 were 9 and 85% of those in the WT, respectively. Most of the mutants grew very slowly or did not grow at all at pH 6.5 or 7.0 in air, and their ability to grow under these conditions was correlated with CO2 uptake capacity. Detailed studies of B1 and M55 indicated that the mutants grew as fast as the WT in liquid at pH 8.0 under air, although they grew poorly on agar plates. The contribution of CO2 uptake appears to be larger on solid medium. Five mutants were constructed by inactivating each of the five ndhD genes in Synechocystis sp. strain PCC 6803. The mutant lacking ndhD3 grew much more slowly than the WT at pH 6.5 under 50 ppm CO2, although other ndhD mutants grew like the WT under these conditions and showed low affinity for CO2 uptake. These results indicated the presence of multiple NAD(P)H dehydrogenase type I complexes with specific roles.

* Corresponding author. Mailing address: Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan. Phone: 81-52-789-5215. Fax: 81-52-789-5214. E-mail: h44975a{at}nucc.cc.nagoya-u.ac.jp.

Journal of Bacteriology, May 2000, p. 2591-2596, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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