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Journal of Bacteriology, May 2000, p. 2639-2642, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The yvaJ Gene of Bacillus subtilis Encodes a 3'-to-5' Exoribonuclease and Is Not Essential in a Strain Lacking Polynucleotide Phosphorylase

Irina A. Oussenko and David H. Bechhofer*

Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine of New York University, New York, New York 10029

Received 4 January 2000/Accepted 9 February 2000

Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major 3'-to-5' exoribonuclease activity in cell extracts, is viable. Here, we show that the B. subtilis yvaJ gene encodes a second 3'-to-5' exoribonuclease. A strain lacking both of these RNases grows slowly but is viable. The existence of another, as yet unknown, 3'-to-5' exoribonuclease in B. subtilis is suggested.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine of New York University, New York, NY 10029. Phone: (212) 241-5628. Fax: (212) 996-7214. E-mail: david.bechhofer{at}mssm.edu.

Journal of Bacteriology, May 2000, p. 2639-2642, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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