This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Regué, M.
Right arrow Articles by Tomás, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Regué, M.
Right arrow Articles by Tomás, J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2001, p. 3564-3573, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3564-3573.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic Characterization of the Klebsiella pneumoniae waa Gene Cluster, Involved in Core Lipopolysaccharide Biosynthesis

Miguel Regué,1,* Núria Climent,1 Nihal Abitiu,1 Núria Coderch,1 Susana Merino,2 Luis Izquierdo,2 Maria Altarriba,2 and Juan M. Tomás2

Departamento de Microbiología y Parasitología Sanitarias, División de Ciéncias de la Salud, Facultad de Farmacia,1 and Departamento de Microbiología, Facultad de Biología,2 Universidad de Barcelona, Barcelona, Spain

Received 22 January 2001/Accepted 4 April 2001

A recombinant cosmid containing genes involved in Klebsiella pneumoniae C3 core lipopolysaccharide biosynthesis was identified by its ability to confer bacteriocin 28b resistance to Escherichia coli K-12. The recombinant cosmid contains 12 genes, the whole waa gene cluster, flanked by kbl and coaD genes, as was found in E. coli K-12. PCR amplification analysis showed that this cluster is conserved in representative K. pneumoniae strains. Partial nucleotide sequence determination showed that the same genes and gene order are found in K. pneumoniae subsp. ozaenae, for which the core chemical structure is known. Complementation analysis of known waa mutants from E. coli K-12 and/or Salmonella enterica led to the identification of genes involved in biosynthesis of the inner core backbone that are shared by these three members of the Enterobacteriaceae. K. pneumoniae orf10 mutants showed a two-log-fold reduction in a mice virulence assay and a strong decrease in capsule amount. Analysis of a constructed K. pneumoniae waaE deletion mutant suggests that the WaaE protein is involved in the transfer of the branch beta -D-Glc to the O-4 position of L-glycero-D-manno-heptose I, a feature shared by K. pneumoniae, Proteus mirabilis, and Yersinia enterocolitica.

* Corresponding author. Mailing address: Departamento de Microbiología y Parasitología Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, Barcelona 08028, Spain. Phone: 34-3-4024496. Fax: 34-3-4021886. E-mail: regue{at}farmacia.far.ub.es.

Journal of Bacteriology, June 2001, p. 3564-3573, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3564-3573.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jimenez, N., Lacasta, A., Vilches, S., Reyes, M., Vazquez, J., Aquillini, E., Merino, S., Regue, M., Tomas, J. M. (2009). Genetics and Proteomics of Aeromonas salmonicida Lipopolysaccharide Core Biosynthesis. J. Bacteriol. 191: 2228-2236 [Abstract] [Full Text]  
  • Jimenez, N., Canals, R., Lacasta, A., Kondakova, A. N., Lindner, B., Knirel, Y. A., Merino, S., Regue, M., Tomas, J. M. (2008). Molecular Analysis of Three Aeromonas hydrophila AH-3 (Serotype O34) Lipopolysaccharide Core Biosynthesis Gene Clusters. J. Bacteriol. 190: 3176-3184 [Abstract] [Full Text]  
  • Lu, G.-T., Ma, Z.-F., Hu, J.-R., Tang, D.-J., He, Y.-Q., Feng, J.-X., Tang, J.-L. (2007). A novel locus involved in extracellular polysaccharide production and virulence of Xanthomonas campestris pathovar campestris. Microbiology 153: 737-746 [Abstract] [Full Text]  
  • Fresno, S., Jimenez, N., Canals, R., Merino, S., Corsaro, M. M., Lanzetta, R., Parrilli, M., Pieretti, G., Regue, M., Tomas, J. M. (2007). A Second Galacturonic Acid Transferase Is Required for Core Lipopolysaccharide Biosynthesis and Complete Capsule Association with the Cell Surface in Klebsiella pneumoniae. J. Bacteriol. 189: 1128-1137 [Abstract] [Full Text]  
  • Fresno, S., Jimenez, N., Izquierdo, L., Merino, S., Corsaro, M. M., De Castro, C., Parrilli, M., Naldi, T., Regue, M., Tomas, J. M. (2006). The ionic interaction of Klebsiella pneumoniae K2 capsule and core lipopolysaccharide. Microbiology 152: 1807-1818 [Abstract] [Full Text]  
  • Canals, R., Jimenez, N., Vilches, S., Regue, M., Merino, S., Tomas, J. M. (2006). The UDP N-Acetylgalactosamine 4-Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence. Infect. Immun. 74: 537-548 [Abstract] [Full Text]  
  • Regue, M., Izquierdo, L., Fresno, S., Jimenez, N., Pique, N., Corsaro, M. M., Parrilli, M., Naldi, T., Merino, S., Tomas, J. M. (2005). The Incorporation of Glucosamine into Enterobacterial Core Lipopolysaccharide: TWO ENZYMATIC STEPS ARE REQUIRED. J. Biol. Chem. 280: 36648-36656 [Abstract] [Full Text]  
  • Frirdich, E., Bouwman, C., Vinogradov, E., Whitfield, C. (2005). The Role of Galacturonic Acid in Outer Membrane Stability in Klebsiella pneumoniae. J. Biol. Chem. 280: 27604-27612 [Abstract] [Full Text]  
  • Regue, M., Izquierdo, L., Fresno, S., Pique, N., Corsaro, M. M., Naldi, T., De Castro, C., Waidelich, D., Merino, S., Tomas, J. M. (2005). A Second Outer-Core Region in Klebsiella pneumoniae Lipopolysaccharide. J. Bacteriol. 187: 4198-4206 [Abstract] [Full Text]  
  • Frirdich, E., Whitfield, C. (2005). Review: Lipopolysaccharide inner core oligosaccharide structure and outer membrane stability in human pathogens belonging to the Enterobacteriaceae. Innate Immunity 11: 133-144 [Abstract]  
  • Edwards, K. J., Allen, S., Gibson, B. W., Campagnari, A. A. (2005). Characterization of a Cluster of Three Glycosyltransferase Enzymes Essential for Moraxella catarrhalis Lipooligosaccharide Assembly. J. Bacteriol. 187: 2939-2947 [Abstract] [Full Text]  
  • Woodford, N., Tierno, P. M. Jr., Young, K., Tysall, L., Palepou, M.-F. I., Ward, E., Painter, R. E., Suber, D. F., Shungu, D., Silver, L. L., Inglima, K., Kornblum, J., Livermore, D. M. (2004). Outbreak of Klebsiella pneumoniae Producing a New Carbapenem- Hydrolyzing Class A {beta}-Lactamase, KPC-3, in a New York Medical Center. Antimicrob. Agents Chemother. 48: 4793-4799 [Abstract] [Full Text]  
  • Frirdich, E., Vinogradov, E., Whitfield, C. (2004). Biosynthesis of a Novel 3-Deoxy-D-manno-oct-2-ulosonic Acid-containing Outer Core Oligosaccharide in the Lipopolysaccharide of Klebsiella pneumoniae. J. Biol. Chem. 279: 27928-27940 [Abstract] [Full Text]  
  • Coderch, N., Pique, N., Lindner, B., Abitiu, N., Merino, S., Izquierdo, L., Jimenez, N., Tomas, J. M., Holst, O., Regue, M. (2004). Genetic and Structural Characterization of the Core Region of the Lipopolysaccharide from Serratia marcescens N28b (Serovar O4). J. Bacteriol. 186: 978-988 [Abstract] [Full Text]  
  • Regue, M., Hita, B., Pique, N., Izquierdo, L., Merino, S., Fresno, S., Benedi, V. J., Tomas, J. M. (2004). A Gene, uge, Is Essential for Klebsiella pneumoniae Virulence. Infect. Immun. 72: 54-61 [Abstract] [Full Text]  
  • Izquierdo, L., Coderch, N., Pique, N., Bedini, E., Corsaro, M. M., Merino, S., Fresno, S., Tomas, J. M., Regue, M. (2003). The Klebsiella pneumoniae wabG Gene: Role in Biosynthesis of the Core Lipopolysaccharide and Virulence. J. Bacteriol. 185: 7213-7221 [Abstract] [Full Text]  
  • Frirdich, E., Lindner, B., Holst, O., Whitfield, C. (2003). Overexpression of the waaZ Gene Leads to Modification of the Structure of the Inner Core Region of Escherichia coli Lipopolysaccharide, Truncation of the Outer Core, and Reduction of the Amount of O Polysaccharide on the Cell Surface. J. Bacteriol. 185: 1659-1671 [Abstract] [Full Text]  
  • Struve, C., Forestier, C., Krogfelt, K. A. (2003). Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection. Microbiology 149: 167-176 [Abstract] [Full Text]  
  • Izquierdo, L., Abitiu, N., Coderch, N., Hita, B., Merino, S., Gavin, R., Tomas, J. M., Regue, M. (2002). The inner-core lipopolysaccharide biosynthetic waaE gene: function and genetic distribution among some Enterobacteriaceae. Microbiology 148: 3485-3496 [Abstract] [Full Text]  
  • Vinogradov, E., Frirdich, E., MacLean, L. L., Perry, M. B., Petersen, B. O., Duus, J. O., Whitfield, C. (2002). Structures of Lipopolysaccharides from Klebsiella pneumoniae. ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS. J. Biol. Chem. 277: 25070-25081 [Abstract] [Full Text]  
  • Valvano, M. A., Messner, P., Kosma, P. (2002). Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology 148: 1979-1989 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»