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Journal of Bacteriology, June 2001, p. 3791-3794, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3791-3794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Red Fluorescent Protein (DsRed) as a Reporter in Saccharomyces cerevisiae

Fernando Rodrigues,1,2 Martijn van Hemert,2 H. Yde Steensma,2,3 Manuela Côrte-Real,1,* and Cecíla Leão1

Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal,1 and Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, 2333 AL Leiden,2 and Kluyver Institute for Biotechnology, Delft University of Technology, 2628 BC Delft,3 The Netherlands

Received 9 January 2001/Accepted 16 March 2001

We describe the utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green fluorescent proteins with both cytoplasmic and nuclear localization were obtained. A series of vectors are now available which can be used to create amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusions with the DsRed protein.

* Corresponding author. Mailing address: Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal. Phone: 351-253-604314. Fax: 351-253-678980. E-mail: mcortereal{at}bio.uminho.pt.

Journal of Bacteriology, June 2001, p. 3791-3794, Vol. 183, No. 12
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.12.3791-3794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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