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Journal of Bacteriology, July 2001, p. 4134-4141, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4134-4141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

OhrR Is a Repressor of ohrA, a Key Organic Hydroperoxide Resistance Determinant in Bacillus subtilis

Mayuree Fuangthong,1 Sopapan Atichartpongkul,2 Skorn Mongkolsuk,2,3 and John D. Helmann1,*

Department of Microbiology, Cornell University, Ithaca, New York 14853-8101,1 and Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210,2 and Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400,3 Thailand

Received 1 February 2001/Accepted 26 April 2001

Bacillus subtilis displays a complex adaptive response to the presence of reactive oxygen species. To date, most proteins that protect against reactive oxygen species are members of the peroxide-inducible PerR and sigma B regulons. We investigated the function of two B. subtilis homologs of the Xanthomonas campestris organic hydroperoxide resistance (ohr) gene. Mutational analyses indicate that both ohrA and ohrB contribute to organic peroxide resistance in B. subtilis, with the OhrA protein playing the more important role in growing cells. Expression of ohrA, but not ohrB, is strongly and specifically induced by organic peroxides. Regulation of ohrA requires the convergently transcribed gene, ohrR, which encodes a member of the MarR family of transcriptional repressors. In an ohrR mutant, ohrA expression is constitutive, whereas expression of the neighboring ohrB gene is unaffected. Selection for mutant strains that are derepressed for ohrA transcription identifies a perfect inverted repeat sequence that is required for OhrR-mediated regulation and likely defines an OhrR binding site. Thus, B. subtilis contains at least three regulons (sigma B, PerR, and OhrR) that contribute to peroxide stress responses.

* Corresponding author. Mailing address: Department of Microbiology, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-6570. Fax: (607) 255-3904. E-mail: jdh9{at}cornell.edu.

Journal of Bacteriology, July 2001, p. 4134-4141, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4134-4141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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