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Journal of Bacteriology, July 2001, p. 4305-4316, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4305-4316.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Autotrophic CO2 Fixation by Chloroflexus aurantiacus: Study of Glyoxylate Formation and Assimilation via the 3-Hydroxypropionate Cycle

Sylvia Herter,1 Jan Farfsing,1 Nasser Gad'On,1 Christoph Rieder,2 Wolfgang Eisenreich,2 Adelbert Bacher,2 and Georg Fuchs1,*

Mikrobiologie, Institut Biologie II, Universität Freiburg, Freiburg,1 and Organische Chemie und Biochemie, Technische Universität München, Munich,2 Germany

Received 12 January 2001/Accepted 20 April 2001

In the facultative autotrophic organism Chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium, the Calvin cycle does not appear to be operative in autotrophic carbon assimilation. An alternative cyclic pathway, the 3-hydroxypropionate cycle, has been proposed. In this pathway, acetyl coenzyme A (acetyl-CoA) is assumed to be converted to malate, and two CO2 molecules are thereby fixed. Malyl-CoA is supposed to be cleaved to acetyl-CoA, the starting molecule, and glyoxylate, the carbon fixation product. Malyl-CoA cleavage is shown here to be catalyzed by malyl-CoA lyase; this enzyme activity is induced severalfold in autotrophically grown cells. Malate is converted to malyl-CoA via an inducible CoA transferase with succinyl-CoA as a CoA donor. Some enzyme activities involved in the conversion of malonyl-CoA via 3-hydroxypropionate to propionyl-CoA are also induced under autotrophic growth conditions. So far, no clue as to the first step in glyoxylate assimilation has been obtained. One possibility for the assimilation of glyoxylate involves the conversion of glyoxylate to glycine and the subsequent assimilation of glycine. However, such a pathway does not occur, as shown by labeling of whole cells with [1,2-13C2]glycine. Glycine carbon was incorporated only into glycine, serine, and compounds that contained C1 units derived therefrom and not into other cell compounds.

* Corresponding author. Mailing address: Mikrobiologie, Institut Biologie II, Schänzlestrasse 1, D-79104 Freiburg, Germany. Phone: 49-761-2032649. Fax: 49-761-2032626. E-mail: fuchsgeo{at}uni-freiburg.de.

Journal of Bacteriology, July 2001, p. 4305-4316, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4305-4316.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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