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Journal of Bacteriology, July 2001, p. 4382-4385, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4382-4385.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Spontaneous Erythromycin Resistance Mutation in a 23S rRNA Gene, rrlA, of the Extreme Thermophile Thermus thermophilus IB-21

Steven T. Gregory,1,* Jamie H. D. Cate,2 and Albert E. Dahlberg1

J. W. Wilson Laboratory, Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912,1 and Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 021422

Received 17 November 2000/Accepted 16 April 2001

Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool for the genetic manipulation of the 23S rRNA genes of Thermus.

* Corresponding author. Mailing address: Box G-J4, J. W. Wilson Laboratory, 69 Brown St., Brown University, Providence, RI 02912. Phone: (401) 863-3652. Fax: (401) 863-1182. E-mail: Steven_Gregory{at}Brown.edu.

Journal of Bacteriology, July 2001, p. 4382-4385, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4382-4385.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gregory, S. T., Dahlberg, A. E. (2009). Genetic and structural analysis of base substitutions in the central pseudoknot of Thermus thermophilus 16S ribosomal RNA. RNA 15: 215-223 [Abstract] [Full Text]  
  • Gregory, S. T., Carr, J. F., Rodriguez-Correa, D., Dahlberg, A. E. (2005). Mutational Analysis of 16S and 23S rRNA Genes of Thermus thermophilus. J. Bacteriol. 187: 4804-4812 [Abstract] [Full Text]  
  • Gregory, S. T., Carr, J. F., Dahlberg, A. E. (2005). A Mutation in the Decoding Center of Thermus thermophilus 16S rRNA Suggests a Novel Mechanism of Streptomycin Resistance. J. Bacteriol. 187: 2200-2202 [Abstract] [Full Text]  
  • Misyurina, O. Y., Chipitsyna, E. V., Finashutina, Y. P., Lazarev, V. N., Akopian, T. A., Savicheva, A. M., Govorun, V. M. (2004). Mutations in a 23S rRNA Gene of Chlamydia trachomatis Associated with Resistance to Macrolides. Antimicrob. Agents Chemother. 48: 1347-1349 [Abstract] [Full Text]  


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