This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, R.
Right arrow Articles by de Montigny, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, R.
Right arrow Articles by de Montigny, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2001, p. 4386-4388, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4386-4388.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants

Renaud Wagner,dagger Marie-Laure Straub, Jean-Luc Souciet, Serge Potier, and Jacky de Montigny*

Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France

Received 12 February 2001/Accepted 19 April 2001

The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, 28, rue Goethe, F-67083 Strasbourg Cedex, France. Phone: 33 3 90 24 18 13. Fax: 33 3 90 24 18 84. E-mail: demontigny{at}gem.u-strasbg.fr.

dagger Present address: UPR9050 du CNRS, ESBS, F-67400 Illkirch, France.

Journal of Bacteriology, July 2001, p. 4386-4388, Vol. 183, No. 14
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.14.4386-4388.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chapeland-Leclerc, F., Bouchoux, J., Goumar, A., Chastin, C., Villard, J., Noel, T. (2005). Inactivation of the FCY2 Gene Encoding Purine-Cytosine Permease Promotes Cross-Resistance to Flucytosine and Fluconazole in Candida lusitaniae. Antimicrob. Agents Chemother. 49: 3101-3108 [Abstract] [Full Text]  
  • Lu, P., Nakorchevskiy, A., Marcotte, E. M. (2003). Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations. Proc. Natl. Acad. Sci. USA 100: 10370-10375 [Abstract] [Full Text]  
  • Poirey, R., Despons, L., Leh, V., Lafuente, M.-J., Potier, S., Souciet, J.-L., Jauniaux, J.-C. (2002). Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability. Microbiology 148: 2111-2123 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»