This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hata, M.
Right arrow Articles by Tanaka, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hata, M.
Right arrow Articles by Tanaka, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2001, p. 4648-4651, Vol. 183, No. 15
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.15.4648-4651.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Involvement of Stringent Factor RelA in Expression of the Alkaline Protease Gene aprE in Bacillus subtilis

Michihiro Hata, Mitsuo Ogura, and Teruo Tanaka*

Department of Marine Science, School of Marine Science and Technology, University of Tokai, Shimizu, Shizuoka 424-8610, Japan

Received 16 April 2001/Accepted 19 April 2001

Expression of Bacillus subtilis aprE, encoding an extracellular alkaline protease, is positively regulated by phosphorylated DegU, the regulator of a two-component regulatory system, DegS-DegU. We found that the expression of an aprE'-'lacZ fusion was greatly reduced in a disruption mutant with a mutation of relA, which encodes the stringent factor RelA. The level of DegU in the relA mutant was similar to that in the wild-type cell. A relA degU double mutation did not result in a further decrease of the aprE'-'lacZ level found in a degU single mutant. The expression of the aprE'-'lacZ fusion in the relA mutant was stimulated by multicopy degR or the degU32(Hy) and degS200(Hy) mutations that cause the stabilization of phosphorylated DegU. Furthermore, the expression of sacB'-'lacZ, which is also dependent on phosphorylated DegU, was stimulated by the relA mutation, and this stimulation was not seen in the relA degU double mutant. These results show that RelA (or its product guanosine-3',5'-bisdiphosphate [pp Gpp]) does not affect the phosphorylation of DegU and suggest that it participates in the expression of aprE and sacB through the regulation of DegU-dependent transcription.

* Corresponding author. Mailing address: Department of Marine Science, School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan. Phone: (543) 34-0411. Fax: (543) 34-9834. E-mail: teruot{at}scc.u-tokai.ac.jp.

Journal of Bacteriology, August 2001, p. 4648-4651, Vol. 183, No. 15
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.15.4648-4651.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Abe, S., Yasumura, A., Tanaka, T. (2009). Regulation of Bacillus subtilis aprE Expression by glnA through Inhibition of scoC and {sigma}D-Dependent degR Expression. J. Bacteriol. 191: 3050-3058 [Abstract] [Full Text]  
  • Kawachi, E., Abe, S., Tanaka, T. (2005). Inhibition of Bacillus subtilis scoC Expression by Multicopy senS. J. Bacteriol. 187: 8526-8530 [Abstract] [Full Text]  
  • Shimane, K., Ogura, M. (2004). Mutational Analysis of the Helix-Turn-Helix Region of Bacillus subtilis Response Regulator DegU, and Identification of cis-Acting Sequences for DegU in the aprE and comK Promoters. J Biochem 136: 387-397 [Abstract] [Full Text]  
  • Ogura, M., Matsuzawa, A., Yoshikawa, H., Tanaka, T. (2004). Bacillus subtilis SalA (YbaL) Negatively Regulates Expression of scoC, Which Encodes the Repressor for the Alkaline Exoprotease Gene, aprE. J. Bacteriol. 186: 3056-3064 [Abstract] [Full Text]  
  • Arai, A., Kawachi, E., Hata, M., Ogura, M., Tanaka, T. (2003). Inhibition of Bacillus subtilis aprE Expression by Lincomycin at the Posttranscriptional Level through Inhibition of ppGpp Synthesis. J Biochem 134: 691-697 [Abstract] [Full Text]  
  • Eymann, C., Homuth, G., Scharf, C., Hecker, M. (2002). Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis. J. Bacteriol. 184: 2500-2520 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»