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Journal of Bacteriology, August 2001, p. 4771-4778, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4771-4778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transduction by phi BB-1, a Bacteriophage of Borrelia burgdorferi

Christian H. Eggers,1,dagger Betsy J. Kimmel,1,Dagger James L. Bono,2,§ Abdallah F. Elias,2 Patricia Rosa,2 and D. Scott Samuels1,*

Division of Biological Sciences, The University of Montana, Missoula, Montana 59812,1 and Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 598402

Received 16 February 2001/Accepted 16 May 2001

We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phi BB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phi BB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phi BB-1 released from this B. burgdorferi strain. phi BB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi.

* Corresponding author. Mailing address: Division of Biological Sciences, The University of Montana, 32 Campus Dr #4824, Missoula, MT 59812-4824. Phone: (406) 243-6145. Fax: (406) 243-4304. E-mail: samuels{at}selway.umt.edu.

dagger Present address: Center for Microbial Pathogenesis, University of Connecticut Health Center, Farmington, CT 06030.

Dagger Present address: Center for Vascular Biology, Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030.

§ Present address: US Meat Animal Research Center, USDA, ARS, Clay Center, NE 68933.

Journal of Bacteriology, August 2001, p. 4771-4778, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4771-4778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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