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Journal of Bacteriology, August 2001, p. 4894-4899, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4894-4899.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Properties of Spores of Bacillus subtilis Blocked at an Intermediate Stage in Spore Germination

Barbara Setlow, Elizabeth Melly, and Peter Setlow*

Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032

Received 28 March 2001/Accepted 30 May 2001

Germination of mutant spores of Bacillus subtilis unable to degrade their cortex is accompanied by excretion of dipicolinic acid and uptake of some core water. However, compared to wild-type germinated spores in which the cortex has been degraded, the germinated mutant spores accumulated less core water, exhibited greatly reduced enzyme activity in the spore core, synthesized neither ATP nor reduced pyridine or flavin nucleotides, and had significantly higher resistance to heat and UV irradiation. We propose that the germinated spores in which the cortex has not been degraded represent an intermediate stage in spore germination, which we term stage I.

* Corresponding author. Mailing address: Department of Biochemistry, MC 3305, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06032. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}sun.uchc.edu.

Journal of Bacteriology, August 2001, p. 4894-4899, Vol. 183, No. 16
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.16.4894-4899.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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