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Journal of Bacteriology, September 2001, p. 5395-5401, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5395-5401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function

Morten Hentzer,1 Gail M. Teitzel,2 Grant J. Balzer,2 Arne Heydorn,1 Søren Molin,1 Michael Givskov,1 and Matthew R. Parsek2,*

Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark,1 and Department of Civil Engineering, Northwestern University, Evanston, Illinois 602082

Received 20 February 2001/Accepted 15 June 2001

During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.

* Corresponding author. Mailing address: Environmental Health Engineering, Department of Civil Engineering, Northwestern University, 2145 Sheridan Rd., Evanston, IL 60208. Phone: (847) 467-7445. Fax: (847) 491-4011. E-mail: m-parsek{at}nwu.edu.

Journal of Bacteriology, September 2001, p. 5395-5401, Vol. 183, No. 18
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.18.5395-5401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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