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Journal of Bacteriology, October 2001, p. 5632-5638, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5632-5638.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rhamnose Biosynthesis Pathway Supplies Precursors for Primary and Secondary Metabolism in Saccharopolyspora spinosa

Krishnamurthy Madduri,* Clive Waldron, and Donald J. Merlo

Dow AgroSciences LLC, Indianapolis, Indiana 46268

Received 14 March 2001/Accepted 12 June 2001

Rhamnose is an essential component of the insect control agent spinosad. However, the genes coding for the four enzymes involved in rhamnose biosynthesis in Saccharopolyspora spinosa are located in three different regions of the genome, all unlinked to the cluster of other genes that are required for spinosyn biosynthesis. Disruption of any of the rhamnose genes resulted in mutants with highly fragmented mycelia that could survive only in media supplemented with an osmotic stabilizer. It appears that this single set of genes provides rhamnose for cell wall synthesis as well as for secondary metabolite production. Duplicating the first two genes of the pathway caused a significant improvement in the yield of spinosyn fermentation products.

* Corresponding author. Mailing address: Dow AgroSciences, 9330 Zionsville Rd., Indianapolis, IN 46268. Phone: (317) 337-3396. Fax: (317) 337-3249. E-mail: kmmadduri{at}dowagro.com.

Journal of Bacteriology, October 2001, p. 5632-5638, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5632-5638.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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