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Journal of Bacteriology, October 2001, p. 5751-5755, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5751-5755.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification, Characterization, and Functional Analysis of a Gene Encoding the Ferric Uptake Regulation Protein in Bartonella Species

Sarah Y. Park,1 Karen L. Kelminson,2 Anthea K. Lee,3 Peng Zhang,2 Rachel E. Warner,2 David H. Rehkopf,2 Stephen B. Calderwood,4,5 and Jane E. Koehler2,*

Division of Infectious Diseases, Departments of Pediatrics1 and Medicine,2 University of California---San Francisco, San Francisco, California 94143-0654; Department of Microbiology and Immunology, Stanford University, Stanford, California 943053; Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts 021144; and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021155

Received 10 April 2001/Accepted 10 July 2001

Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and other Fur proteins was a substantially higher predicted isoelectric point. No promoter activity or Fur autoregulation was detected using a gfp reporter gene fused to the 204 nucleotides immediately upstream of the Bartonella fur gene. Bartonella henselae fur gene expression complemented a Vibrio cholerae fur mutant.

* Corresponding author. Mailing address: Division of Infectious Diseases, 521 Parnassus Ave., Room C-443, University of California---San Francisco, San Francisco, California 94143-0654. Phone: (415) 476-3536. Fax: (415) 476-9364. E-mail: jkoehler{at}medicine.ucsf.edu.

Journal of Bacteriology, October 2001, p. 5751-5755, Vol. 183, No. 19
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.19.5751-5755.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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