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Journal of Bacteriology, January 2001, p. 644-653, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.644-653.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcription of nhaA, the Main Na+/H+ Antiporter of Escherichia coli, Is Regulated by Na+ and Growth Phase

Nir Dover and Etana Padan*

Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

Received 7 August 2000/Accepted 19 October 2000

The transcription of nhaA, encoding the main Na+/H+ antiporter of Escherichia coli, is induced by Na+, regulated by NhaR, and affected by H-NS. In this work the roles of the two nhaA promoters (P1 and P2) were studied by analysis of transcription both in vivo and in vitro and promoter mutations. We found that P1 is an NhaR-dependent, Na+-induced, and H-NS-affected promoter both in the exponential and stationary phases. An in vitro transcription assay demonstrated that P1 is activated by sigma 70-RNA polymerase and both NhaR and H-NS increase the specificity of P1. Remarkably, in marked contrast to P1, P2 exhibits very low activity during the exponential phase but is induced in the stationary phase to become the major promoter. Furthermore, P2 is activated by sigma S and is neither induced by Na+ nor dependent on NhaR or affected by H-NS. Hence, this work establishes that nhaA has a dual mode of regulation, each involving a different promoter, and reveals that P2 and sigma S together are responsible for the survival of stationary-phase cells in the presence of high Na+, alkaline pH, and the combination of high Na+ and alkaline pH, the most stressful condition.

* Corresponding author. Mailing address: Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel. Phone: 972-2-6585094. Fax: 972-2-6586947. E-mail: etana{at}vms.huji.ac.il.

Journal of Bacteriology, January 2001, p. 644-653, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.644-653.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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