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Journal of Bacteriology, January 2001, p. 664-670, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.664-670.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Two-Component System That Regulates Methanol and Formaldehyde Oxidation in Paracoccus denitrificans

N. Harms,* W. N. M. Reijnders, S. Koning, and R. J. M. van Spanning

Department of Molecular Cell Physiology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

Received 10 April 2000/Accepted 29 October 2000

A chromosomal region encoding a two-component regulatory system, FlhRS, has been isolated from Paracoccus denitrificans. FlhRS-deficient mutants were unable to grow on methanol, methylamine, or choline as the carbon and energy source. Expression of the gene encoding glutathione-dependent formaldehyde dehydrogenase (fhlA) was undetectable in the mutant, and expression of the S-formylglutathione hydrolase gene (fghA) was reduced in the mutant background. In addition, methanol dehydrogenase was immunologically undetectable in cell extracts of FhlRS mutants. These results indicate that the FlhRS sensor-regulator pair is involved in the regulation of formaldehyde, methanol, and methylamine oxidation. The effect that the FlhRS proteins exert on the regulation of C1 metabolism might be essential to maintain the internal concentration of formaldehyde below toxic levels.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands. Phone: 31-204447176. Fax: 31-204447136. E-mail: harms{at}bio.vu.nl.

Journal of Bacteriology, January 2001, p. 664-670, Vol. 183, No. 2
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.2.664-670.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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