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Journal of Bacteriology, October 2001, p. 6036-6045, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6036-6045.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

SseBCD Proteins Are Secreted by the Type III Secretion System of Salmonella Pathogenicity Island 2 and Function as a Translocon

Thomas Nikolaus,1 Jörg Deiwick,1 Catherine Rappl,1 Jeremy A. Freeman,2 Werner Schröder,3 Samuel I. Miller,2,4 and Michael Hensel1,*

Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, Munich,1 and Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Berlin,3 Germany, and Departments of Microbiology2 and Medicine,4 University of Washington, Seattle, Washington

Received 24 April 2001/Accepted 26 June 2001

The type III secretion system encoded by Salmonella pathogenicity island 2 (SPI2) is required for systemic infections and intracellular accumulation of Salmonella enterica. This system is induced by intracellular Salmonella and subsequently transfers effector proteins into the host cell. Growth conditions either inducing expression of the type III secretion system or the secretion of substrate proteins were defined. Here we report the identification of a set of substrate proteins consisting of SseB, SseC, and SseD that are secreted by the SPI2 system in vitro. Secretion was observed if bacterial cells were exposed to acidic pH after growth in minimal medium with limitation of Mg2+ or phosphate. SseB, -C, and -D were isolated in a fraction detached from the bacterial cell surface by mechanical shearing, indicating that these proteins are predominantly assembled into complexes on the bacterial cell surface. The three proteins were required for the translocation of SPI2 effector proteins SspH1 and SspH2 into infected host cells. Thus, SseB, SseC, and SseD function as the translocon for effector proteins by intracellular Salmonella.

* Corresponding author. Mailing address: Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich-Alexander-Universität Erlangen-Nürnberg, Wasserturmstr. 3/5, D-91054 Erlangen, Germany. Phone: 49 (0) 9131 85 23640. Fax: 49 (0) 9131 85 22531. E-mail: hensel{at}mikrobio.med.uni-erlangen.de.

Journal of Bacteriology, October 2001, p. 6036-6045, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6036-6045.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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