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Journal of Bacteriology, October 2001, p. 6140-6143, Vol. 183, No. 20
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.20.6140-6143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Novel Motility Mutants of
Synechocystis Strain PCC 6803 Generated by In Vitro
Transposon Mutagenesis
Devaki
Bhaya,1,*
Akiko
Takahashi,2
Payam
Shahi,3 and
Arthur R.
Grossman1
Department of Plant Biology, Carnegie
Institution, Stanford, California 943051;
Riken Plant Science Center, Laboratory for Metabolic
Compartmentation, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan2; and Department of Biological
Sciences, San Jose State University, San Jose, California
951923
Received 18 May 2001/Accepted 13 July 2001
We screened for transposon-generated mutants of
Synechocystis sp. strain PCC 6803 that exhibited
aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in
genes encoding chemotaxis-related proteins, while others mapped
to novel genes. These novel genes and their possible roles in motility
are discussed.
*
Corresponding author. Mailing address: Department of
Plant Biology, Carnegie Institution, 260 Panama Street, Stanford, CA 94305. Phone: (650) 325-1521. Fax: (650) 325-6857. E-mail:
devaki{at}andrew2.stanford.edu.

Carnegie Institution of Washington publication no.
1488.
Journal of Bacteriology, October 2001, p. 6140-6143, Vol. 183, No. 20
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.20.6140-6143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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