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Journal of Bacteriology, October 2001, p. 6140-6143, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6140-6143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Motility Mutants of Synechocystis Strain PCC 6803 Generated by In Vitro Transposon Mutagenesis†

Devaki Bhaya,1,* Akiko Takahashi,2 Payam Shahi,3 and Arthur R. Grossman1

Department of Plant Biology, Carnegie Institution, Stanford, California 943051; Riken Plant Science Center, Laboratory for Metabolic Compartmentation, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan2; and Department of Biological Sciences, San Jose State University, San Jose, California 951923

Received 18 May 2001/Accepted 13 July 2001

We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in genes encoding chemotaxis-related proteins, while others mapped to novel genes. These novel genes and their possible roles in motility are discussed.


* Corresponding author. Mailing address: Department of Plant Biology, Carnegie Institution, 260 Panama Street, Stanford, CA 94305. Phone: (650) 325-1521. Fax: (650) 325-6857. E-mail: devaki{at}andrew2.stanford.edu.

dagger Carnegie Institution of Washington publication no. 1488.


Journal of Bacteriology, October 2001, p. 6140-6143, Vol. 183, No. 20
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.20.6140-6143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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