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Journal of Bacteriology, November 2001, p. 6282-6287, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6282-6287.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Heat-Shock-Induced Proteins from Myxococcus xanthus

Mieko Otani,1 Junko Tabata,1 Toshiyuki Ueki,2 Keiji Sano,1 and Sumiko Inouye2,*

Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Nishi-ku, Kobe 651-2180, Japan,1 and Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 088542

Received 14 June 2001/Accepted 7 August 2001

Optimal conditions for two-dimensional gel electrophoresis of total cellular proteins from Myxococcus xanthus were established. Using these conditions, we analyzed protein patterns of heat-shocked M. xanthus cells. Eighteen major spots and 15 minor spots were found to be induced by heat shock. From N-terminal sequences of 15 major spots, DnaK, GroEL, GroES, alkyl hydroperoxide reductase, aldehyde dehydrogenase, succinyl coenzyme A (CoA) synthetase, 30S ribosomal protein S6, and ATP synthase alpha  subunit were identified. Three of the 18 major spots had an identical N-terminal sequence, indicating that they may be different forms of the same protein. Although a DnaK homologue, SglK, has been identified in M. xanthus (R. M. Weimer, C. Creghton, A. Stassinopoulos, P. Youderian, and P. L. Hartzell, J. Bacteriol. 180:5357-5368, 1998; Z. Yang, Y. Geng, and W. Shi, J. Bacteriol. 180:218-224, 1998), SglK was not induced by heat shock. In addition, there were seven substitutions within the N-terminal 30-residue sequence of the newly identified DnaK. This is the first report to demonstrate that succinyl CoA synthetase, 30S ribosomal protein S6, and ATP synthase alpha  subunit are heat shock inducible.

* Corresponding author. Mailing address: Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Ln., Piscataway, NJ 08854. Phone: (732) 235-4161. Fax: (732) 235-4559. E-mail: sinouye{at}waksman.rutgers.edu.

Journal of Bacteriology, November 2001, p. 6282-6287, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6282-6287.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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