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Journal of Bacteriology, November 2001, p. 6429-6434, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6429-6434.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The coxBAC Operon Encodes a Cytochrome c Oxidase Required for Heterotrophic Growth in the Cyanobacterium Anabaena variabilis Strain ATCC 29413

Georg Schmetterer,1,* Ana Valladares,2 Dietmar Pils,1 Susanne Steinbach,1 Margit Pacher,1 Alicia M. Muro-Pastor,2 Enrique Flores,2 and Antonia Herrero2

Membran Protein Gruppe, Institut für Physikalische Chemie der Universität Wien, A-1090 Vienna, Austria,1 and Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC---Universidad de Sevilla, E-41092 Seville, Spain2

Received 22 February 2001/Accepted 7 August 2001

Three genes, coxB, coxA, and coxC, found in a clone from a gene library of the cyanobacterium Anabaena variabilis strain ATCC 29413, were identified by hybridization with an oligonucleotide specific for aa3-type cytochrome c oxidases. Deletion of these genes from the genome of A. variabilis strain ATCC 29413 FD yielded strain CSW1, which displayed no chemoheterotrophic growth and an impaired cytochrome c oxidase activity. Photoautotrophic growth of CSW1, however, was unchanged, even with dinitrogen as the nitrogen source. A higher cytochrome c oxidase activity was detected in membrane preparations from dinitrogen-grown CSW1 than from nitrate-grown CSW1, but comparable activities of respiratory oxygen uptake were found in the wild type and in CSW1. Our data indicate that the identified cox gene cluster is essential for fructose-dependent growth in the dark, but not for growth on dinitrogen, and that other terminal respiratory oxidases are expressed in this cyanobacterium. Transcription analysis showed that coxBAC constitutes an operon which is expressed from two transcriptional start points. The use of one of them was stimulated by fructose.

* Corresponding author. Mailing address: Institute of Physical Chemistry, UZA2, Althanstrasse 14, A-1090 Vienna, Austria. Phone: 43-1-4277-52548. Fax: 43-1-4277-52546. E-mail: georg.schmetterer{at}univie.ac.at.

Journal of Bacteriology, November 2001, p. 6429-6434, Vol. 183, No. 21
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.21.6429-6434.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Valladares, A., Maldener, I., Muro-Pastor, A. M., Flores, E., Herrero, A. (2007). Heterocyst Development and Diazotrophic Metabolism in Terminal Respiratory Oxidase Mutants of the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 4425-4430 [Abstract] [Full Text]  
  • Jones, K. M., Haselkorn, R. (2002). Newly Identified Cytochrome c Oxidase Operon in the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC 7120 Specifically Induced in Heterocysts. J. Bacteriol. 184: 2491-2499 [Abstract] [Full Text]  


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