This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blank, L. M.
Right arrow Articles by Jensen, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blank, L. M.
Right arrow Articles by Jensen, P. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2001, p. 6707-6709, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6707-6709.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hemin Reconstitutes Proton Extrusion in an H+-ATPase-Negative Mutant of Lactococcus lactis

Lars M. Blank,1 Brian J. Koebmann,2 Ole Michelsen,2 Lars K. Nielsen,1 and Peter R. Jensen2,*

Department of Chemical Engineering, University of Queensland, 4072 Brisbane, Australia,1 and Section of Molecular Microbiology, BioCentrum, Technical University of Denmark, DK-2800 Lyngby, Denmark2

Received 30 May 2001/Accepted 20 August 2001

H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

* Corresponding author. Mailing address: Section of Molecular Microbiology, BioCentrum, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark. Phone: 45 45-252510. Fax: 45 45-932809. E-mail: Peter.R.Jensen{at}BioCentrum.DTU.DK.

Journal of Bacteriology, November 2001, p. 6707-6709, Vol. 183, No. 22
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.22.6707-6709.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pedersen, M. B., Garrigues, C., Tuphile, K., Brun, C., Vido, K., Bennedsen, M., Mollgaard, H., Gaudu, P., Gruss, A. (2008). Impact of Aeration and Heme-Activated Respiration on Lactococcus lactis Gene Expression: Identification of a Heme-Responsive Operon. J. Bacteriol. 190: 4903-4911 [Abstract] [Full Text]  
  • Brooijmans, R. J. W., Poolman, B., Schuurman-Wolters, G. K., de Vos, W. M., Hugenholtz, J. (2007). Generation of a Membrane Potential by Lactococcus lactis through Aerobic Electron Transport. J. Bacteriol. 189: 5203-5209 [Abstract] [Full Text]  
  • Vido, K., le Bars, D., Mistou, M.-Y., Anglade, P., Gruss, A., Gaudu, P. (2004). Proteome Analyses of Heme-Dependent Respiration in Lactococcus lactis: Involvement of the Proteolytic System. J. Bacteriol. 186: 1648-1657 [Abstract] [Full Text]  
  • Sybesma, W., Starrenburg, M., Tijsseling, L., Hoefnagel, M. H. N., Hugenholtz, J. (2003). Effects of Cultivation Conditions on Folate Production by Lactic Acid Bacteria. Appl. Environ. Microbiol. 69: 4542-4548 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»