This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Castañeda, M.
Right arrow Articles by Espín, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castañeda, M.
Right arrow Articles by Espín, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2001, p. 6787-6793, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6787-6793.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Global Regulators GacA and sigma S Form Part of a Cascade That Controls Alginate Production in Azotobacter vinelandii

Miguel Castañeda, Judith Sánchez, Soledad Moreno, Cinthia Núñez, and Guadalupe Espín*

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca Morelos 62250, Mexico

Received 26 June 2001/Accepted 12 September 2001

Transcription of the Azotobacter vinelandii algD gene, which encodes GDP-mannose dehydrogenase (the rate-limiting enzyme of alginate synthesis), starts from three sites: p1, p2, and p3. The sensor kinase GacS, a member of the two-component regulatory system, is required for transcription of algD from its three sites during the stationary phase. Here we show that algD is expressed constitutively throughout the growth cycle from the p2 and p3 sites and that transcription from p1 started at the transition between the exponential growth phase and stationary phase. We constructed A. vinelandii strains that carried mutations in gacA encoding the cognate response regulator of GacS and in rpoS coding for the stationary-phase sigma S factor. The gacA mutation impaired alginate production and transcription of algD from its three promoters. Transcription of rpoS was also abolished by the gacA mutation. The rpoS mutation impaired transcription of algD from the p1 promoter and increased it from the p2 sigma E promoter. The results of this study provide evidence for the predominant role of GacA in a regulatory cascade controlling alginate production and gene expression during the stationary phase in A. vinelandii.

* Corresponding author. Mailing address: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca Morelos 62250, Mexico. Phone: 52-73-291644. Fax: 52-73-172388. E-mail: espin{at}ibt.unam.mx.

Journal of Bacteriology, December 2001, p. 6787-6793, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6787-6793.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Setubal, J. C., dos Santos, P., Goldman, B. S., Ertesvag, H., Espin, G., Rubio, L. M., Valla, S., Almeida, N. F., Balasubramanian, D., Cromes, L., Curatti, L., Du, Z., Godsy, E., Goodner, B., Hellner-Burris, K., Hernandez, J. A., Houmiel, K., Imperial, J., Kennedy, C., Larson, T. J., Latreille, P., Ligon, L. S., Lu, J., Maerk, M., Miller, N. M., Norton, S., O'Carroll, I. P., Paulsen, I., Raulfs, E. C., Roemer, R., Rosser, J., Segura, D., Slater, S., Stricklin, S. L., Studholme, D. J., Sun, J., Viana, C. J., Wallin, E., Wang, B., Wheeler, C., Zhu, H., Dean, D. R., Dixon, R., Wood, D. (2009). Genome Sequence of Azotobacter vinelandii, an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes. J. Bacteriol. 191: 4534-4545 [Abstract] [Full Text]  
  • Morash, M. G., Brassinga, A. K. C., Warthan, M., Gourabathini, P., Garduno, R. A., Goodman, S. D., Hoffman, P. S. (2009). Reciprocal Expression of Integration Host Factor and HU in the Developmental Cycle and Infectivity of Legionella pneumophila. Appl. Environ. Microbiol. 75: 1826-1837 [Abstract] [Full Text]  
  • Nunez, C., Bogachev, A. V., Guzman, G., Tello, I., Guzman, J., Espin, G. (2009). The Na+-translocating NADH : ubiquinone oxidoreductase of Azotobacter vinelandii negatively regulates alginate synthesis. Microbiology 155: 249-256 [Abstract] [Full Text]  
  • Sandercock, J. R., Page, W. J. (2008). RpoS Expression and the General Stress Response in Azotobacter vinelandii during Carbon and Nitrogen Diauxic Shifts. J. Bacteriol. 190: 946-953 [Abstract] [Full Text]  
  • Bertani, I., Venturi, V. (2004). Regulation of the N-Acyl Homoserine Lactone-Dependent Quorum-Sensing System in Rhizosphere Pseudomonas putida WCS358 and Cross-Talk with the Stationary-Phase RpoS Sigma Factor and the Global Regulator GacA. Appl. Environ. Microbiol. 70: 5493-5502 [Abstract] [Full Text]  
  • Berleman, J. E., Hasselbring, B. M., Bauer, C. E. (2004). Hypercyst Mutants in Rhodospirillum centenum Identify Regulatory Loci Involved in Cyst Cell Differentiation. J. Bacteriol. 186: 5834-5841 [Abstract] [Full Text]  
  • Whistler, C. A., Ruby, E. G. (2003). GacA Regulates Symbiotic Colonization Traits of Vibrio fischeri and Facilitates a Beneficial Association with an Animal Host. J. Bacteriol. 185: 7202-7212 [Abstract] [Full Text]  
  • Peralta-Gil, M., Segura, D., Guzman, J., Servin-Gonzalez, L., Espin, G. (2002). Expression of the Azotobacter vinelandii Poly-{beta}-Hydroxybutyrate Biosynthetic phbBAC Operon Is Driven by Two Overlapping Promoters and Is Dependent on the Transcriptional Activator PhbR. J. Bacteriol. 184: 5672-5677 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»